Survival and Proliferation under Severely Hypoxic Microenvironments Using Cell-Laden Oxygenating Hydrogels
| Autor: | Berivan Cecen, Anderson Oliveira Lobo, Shikha Sebastian, Fernanda Roberta Marciano, R. Peña-Garcia, Amir K. Miri, Ali Fattahi, Hamza Zaidi, Shabir Hassan, Christina Karavasili |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
Materials science food.ingredient Necrosis Cell Biomedical Engineering chemistry.chemical_element 02 engineering and technology 010402 general chemistry 01 natural sciences Oxygen Gelatin Article chemistry.chemical_compound food R5-920 In vivo Calcium peroxide medicine sever hypoxia hydrogels chemistry.chemical_classification microparticles Reactive oxygen species 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure chemistry Self-healing hydrogels Biophysics cells medicine.symptom 0210 nano-technology electrospray oxygen TP248.13-248.65 biomaterials Biotechnology |
| Zdroj: | Journal of Functional Biomaterials, Vol 12, Iss 30, p 30 (2021) Journal of Functional Biomaterials Volume 12 Issue 2 Pages: 30 |
| ISSN: | 2079-4983 |
| Popis: | Different strategies have been employed to provide adequate nutrients for engineered living tissues. These have mainly revolved around providing oxygen to alleviate the effects of chronic hypoxia or anoxia that result in necrosis or weak neovascularization, leading to failure of artificial tissue implants and hence poor clinical outcome. While different biomaterials have been used as oxygen generators for in vitro as well as in vivo applications, certain problems have hampered their wide application. Among these are the generation and the rate at which oxygen is produced together with the production of the reaction intermediates in the form of reactive oxygen species (ROS). Both these factors can be detrimental for cell survival and can severely affect the outcome of such studies. Here we present calcium peroxide (CPO) encapsulated in polycaprolactone as oxygen releasing microparticles (OMPs). While CPO releases oxygen upon hydrolysis, PCL encapsulation ensures that hydrolysis takes place slowly, thereby sustaining prolonged release of oxygen without the stress the bulk release can endow on the encapsulated cells. We used gelatin methacryloyl (GelMA) hydrogels containing these OMPs to stimulate survival and proliferation of encapsulated skeletal myoblasts and optimized the OMP concentration for sustained oxygen delivery over more than a week. The oxygen releasing and delivery platform described in this study opens up opportunities for cell-based therapeutic approaches to treat diseases resulting from ischemic conditions and enhance survival of implants under severe hypoxic conditions for successful clinical translation. |
| Databáze: | OpenAIRE |
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