Identification of two histidines necessary for reovirus mRNA guanylyltransferase activity
| Autor: | Tao Qiu, Cindy L. Luongo |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Guanylyltransferase
Carps food.ingredient MRNA guanylyltransferase activity Molecular Sequence Data Reoviridae Substrate Specificity food mRNA guanylyltransferase Chlorides Virology Animals Aquareovirus Histidine Amino Acid Sequence Orthoreovirus biology Sulfates Hydrogen-Ion Concentration biology.organism_classification Nucleotidyltransferases Molecular biology Biochemistry Guanylyltransferase activity Capsid Proteins Guanosine Triphosphate |
| Zdroj: | Virology. 316(2):313-324 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2003.08.027 |
| Popis: | Grass carp reovirus, a segmented double-stranded RNA virus, is a member of the genus aquareovirus in the Reoviridae family. Grass carp reovirus VP1 was shown to be an mRNA guanylyltransferase. The enzyme demonstrated maximum activityor= pH 6.0. This low pH maximum is conserved among the known guanylyltransferases of the Reoviridae family, but is not a property of the KxDG guanylyltransferases. The positive effect of low pH was detected for both autoguanylylation and GMP transfer, the two steps in the guanylyltransferase reaction. The effect of pH on enzymatic activity suggested that histidine protonation is responsible for the observed increase in guanylyltransferase activity. Mutagenesis of the two histidines conserved among the orthoreovirus and aquareovirus guanylyltransferases demonstrated that they are necessary for activity. |
| Databáze: | OpenAIRE |
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