Cell line specific modulation of connexin43 expression after exposure to ionizing radiation
| Autor: | George Iliakis, Ferya Banaz-Yaşar, Rabea Tischka, Elke Winterhager, Alexandra Gellhaus |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Clinical Biochemistry
Medizin Endogeny Biology Transfection Cell Line Mice Downregulation and upregulation Cell Line Tumor Bystander effect Animals Humans RNA Messenger Gap junction Endothelial Cells Gap Junctions Cell Biology General Medicine Bystander Effect Cell biology Endothelial stem cell Gene Expression Regulation Cell culture Connexin 43 cardiovascular system sense organs biological phenomena cell phenomena and immunity Intracellular |
| Popis: | Gap junctional intercellular communication plays a significant role in mediating radiation-induced bystander effects. However, the level of Cx43 itself is influenced by ionizing radiation, which could modify the bystander effect. Here we have investigated several cell lines for the modulation of Cx43 expression 24 h after irradiation with 5 Gy X-rays. The mouse endothelial cell line bEnd3 revealed a significantly elevated level of Cx43 already 15 min after exposure to X-rays, whereas human hybrid endothelial cells (EA.hy926) exhibited a transient downregulation of Cx43 mRNA. No obvious changes in the communication properties of the different cell lines could be observed after irradiation. The communication-deficient malignant human trophoblast cell line Jeg3 stably transfected with Cx43 did not reveal any induction of endogenous nor alteration in the exogenous Cx43 transcript level upon exposure to 5 Gy. Taken together, our data show a cell line specific modulation of Cx43 expression after exposure to X-rays. |
| Databáze: | OpenAIRE |
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