Biglycan expression in hypertensive subjects with normal or increased carotid intima-media wall thickness
| Autor: | Stefania Riggio, Egidio Imbalzano, Antonino Saitta, M.A. Sardo, Carlo Saitta, Salvatore Campo, Alessandra Bitto, Giuseppe Mandraffino, Angela Alibrandi |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Adult
Lipopolysaccharides Male medicine.medical_specialty Clinical Biochemistry Inflammation Essential hypertension Biochemistry Monocytes Basal (phylogenetics) Internal medicine Biglycan medicine Humans RNA Messenger Interleukin 6 Extracellular Matrix Proteins biology business.industry Interleukin-6 Tumor Necrosis Factor-alpha Monocyte Angiotensin II Macrophages Biochemistry (medical) General Medicine medicine.disease Endocrinology medicine.anatomical_structure C-Reactive Protein Carotid Arteries Intima-media thickness Gene Expression Regulation Case-Control Studies Hypertension biology.protein Regression Analysis Female Proteoglycans medicine.symptom business Tunica Intima Arterial hypertension Carotid intima media thickness Atherosclerosis |
| Popis: | Biglycan (BGN), an extracellular matrix proteoglycan, has been shown to convey pro-inflammatory signals. In the present study we investigated BGN expression and its correlation with plasma levels of inflammatory markers in hypertensive subjects with or without alteration of carotid intima media thickness (IMT).We evaluated 123 untreated essential hypertensives with no additional risk factors for atherosclerosis nor signs of cardiovascular disease and 40 controls. Hypertensives were classified according to a normal (or =1 mm) or increased (1 mm) IMT. BGN-mRNA and protein expression were measured in unstimulated, LPS- and Angiotensin II (Ang-II)-stimulated blood monocytes. Plasma concentrations of interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and high sensitivity-C-reactive protein (hs-CRP) were also measured.We found increased levels of IL-6, TNF-alpha, hs-CRP, and BGN-mRNA and protein in hypertensives vs controls (1.72+/-0.60 vs 1 n-fold, and 3.60+/-0.75 vs 1 n-fold, both p0.001). However, BGN expression was not significantly different between hypertensives with IMTor =1 mm and1 mm. Furthermore, in vitro addition of Ang II enhanced basal BGN-mRNA (in hypertensives: 3.57+/-1.08 vs 1.72+/-0.60 n-fold, p0.001) and protein (in hypertensives: 4.92+/-0.42 vs 3.41+/-0.75, p0.001) expression in monocytes.Our data provide evidence of an enhanced expression of BGN in essential hypertension. In addition we suggest that Ang II can mediate monocyte BGN production. |
| Databáze: | OpenAIRE |
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