Expression of myeloid differentiation antigens in acute nonlymphocytic leukemia: increased concentration of CD33 antigen predicts poor outcome--a report from the Childrens Cancer Study Group
| Autor: | G. Denman Hammond, Sergio Piomelli, John H. Kersey, Mark E. Nesbit, Stephen A. Feig, Jonathan D. Buckley, Beatrice Lampkin, Irwin D. Bernstein, Patricia A. Dinndorf |
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| Rok vydání: | 1992 |
| Předmět: |
Cancer Research
Myeloid medicine.drug_class CD33 Sialic Acid Binding Ig-like Lectin 3 Antigens Differentiation Myelomonocytic Fluorescent Antibody Technique Gene Expression Monoclonal antibody Immunophenotyping Antigen Antigens CD hemic and lymphatic diseases Medicine Humans Child biology business.industry Remission Induction Cancer Antibodies Monoclonal medicine.disease Prognosis Survival Analysis Leukemia Leukemia Myeloid Acute medicine.anatomical_structure Phenotype Oncology Pediatrics Perinatology and Child Health Immunology Multivariate Analysis biology.protein Antibody business Follow-Up Studies |
| Zdroj: | Medical and pediatric oncology. 20(3) |
| ISSN: | 0098-1532 |
| Popis: | Ninety-eight cryopreserved specimens of acute nonlymphocytic leukemia (ANLL) cells obtained at initial diagnosis of children enrolled on the Childrens Cancer Study Group 251 protocol (CCG 251) were examined by indirect immunofluorescence using four monoclonal antibodies to myeloid differentiation antigens. The relationship between the level of differentiation of ANLL cells as determined by their antigen phenotype and the clinical outcome of treatment, including complete remission (CR) rate, survival, and event-free survival, was evaluated. Most leukemic specimens were determined to express the CD33 antigen (L4F3), a 67-kD protein. Because the level of differentiation of normal myeloid cells is reflected by the concentration of the CD33 antigen expressed, samples were categorized as CD33-bright (immature) versus CD33-dull (mature). Patients with CD33-bright leukemic blasts had a marginally inferior CR rate to those with CD33-dull blasts (P = 0.08). With respect to survival and event-free survival, there was a significantly inferior outcome in the CD33-bright patients (P = 0.04 and P = 0.06, respectively). Reactions of ANLL with anti-CD15 antibody (1G10), anti-CD36 antibody (5F1), or anti-CD17 antibody (T5A7) did not predict clinical outcome. This study indicates that patients whose ANLL blasts displayed the CD33 antigen in an amount associated with immature myeloid cells experienced a worse outcome than patients with ANLL blasts that expressed a phenotype associated with more mature cells. © 1992 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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