Divergence in species and regulatory role of β-myosin heavy chain proximal promoter muscle-CAT elements
| Autor: | Yangsi Ou, Natalia G. Karasseva, John J. McCarthy, Richard W. Tsika, Gretchen L. Tsika |
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| Rok vydání: | 2002 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Physiology Transgene Response element Mice Transgenic Biology Muscle hypertrophy Ventricular Myosins Weight-Bearing Chloramphenicol acetyltransferase Mice Species Specificity Transcription (biology) Myosin Animals Humans Muscle Skeletal Promoter Regions Genetic Cell Nucleus Genetics Proximal promoter Myosin Heavy Chains Basic Helix-Loop-Helix Leucine Zipper Transcription Factors Nucleotides Nuclear Proteins TEA Domain Transcription Factors DNA Organ Size Cell Biology In vitro Rats Cell biology DNA-Binding Proteins Basic-Leucine Zipper Transcription Factors Mutation Transcription Factors |
| Zdroj: | American Journal of Physiology-Cell Physiology. 283:C1761-C1775 |
| ISSN: | 1522-1563 0363-6143 |
| DOI: | 10.1152/ajpcell.00278.2002 |
| Popis: | We examined the functional role of distinct muscle-CAT (MCAT) elements during non-weight-bearing (NWB) regulation of a wild-type 293-base pair β-myosin heavy chain (βMyHC) transgene. Electrophoretic mobility shift assays (EMSA) revealed decreased NTEF-1, poly(ADP-ribose) polymerase, and Max binding at the human distal MCAT element when using NWB soleus vs. control soleus nuclear extract. Compared with the wild-type transgene, expression assays revealed that distal MCAT element mutation decreased basal transgene expression, which was decreased further in response to NWB. EMSA analysis of the human proximal MCAT (pMCAT) element revealed low levels of NTEF-1 binding that did not differ between control and NWB extract, whereas the rat pMCAT element displayed robust NTEF-1 binding that decreased when using NWB soleus extracts. Differences in binding between human and rat pMCAT elements were consistent whether using rat or mouse nuclear extract or in vitro synthesized human TEF-1 proteins. Our results provide the first evidence that 1) different binding properties and likely regulatory functions are served by the human and rat pMCAT elements, and 2) previously unrecognized βMyHC proximal promoter elements contribute to NWB regulation. |
| Databáze: | OpenAIRE |
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