MicroRNA-126 Reduces Blood-Retina Barrier Breakdown via the Regulation of VCAM-1 and BCL2L11 in Ischemic Retinopathy
Autor: | Yanyan Bai, Xia Bai, Jingcheng Miao, Junpeng Luo, Xiaofeng Zhang, Zhaoyue Wang, Jingjing Han |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Blotting Western Blood–retinal barrier Vascular Cell Adhesion Molecule-1 Apoptosis Vascular permeability Biology Retina Capillary Permeability Mice 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Retinal Diseases Ischemia Blood-Retinal Barrier medicine Animals VCAM-1 Evans Blue Bcl-2-Like Protein 11 Tight junction Endothelial Cells Retinal Vessels Retinal General Medicine medicine.disease Sensory Systems Cell biology Mice Inbred C57BL Disease Models Animal MicroRNAs Ophthalmology 030104 developmental biology medicine.anatomical_structure Animals Newborn chemistry Immunology sense organs Retinopathy |
Zdroj: | Ophthalmic Research. 57:173-185 |
ISSN: | 1423-0259 0030-3747 |
Popis: | To evaluate the role of microRNA-126 (miR-126) in maintaining the integrity of the blood-retina barrier (BRB), we established a mouse model of oxygen-induced retinopathy (OIR) and measured the retinal levels of miR-126 using recombinant plasmid pCMV-MIR or pCMV-MIR-126 intravitreal injections. We also detected VCAM-1 and BCL2L11 levels. Retinal vaso-obliteration, VCAM-1 localization on retinal endothelial cells, the blood-retina vascular permeability or albumin leakage in retinas, TUNEL histology, Evans blue assays, or Western blotting for detecting albumin or tight junction levels in the retina was performed. We also detected the effect of miR-126 on the survival of Müller cells in a mouse model using vimentin fluorescence staining. Our results suggested that miR-126 may not only regulate the overexpression of VCAM-1 or BCL2L11 and lead to the reduction of retinal endothelial cell apoptosis, retinal vascular leakage, or retinal permeability in the OIR mouse model, but may also protect hypoxic retinal Müller cells via the STAT3 signaling pathway. We believe that miR-126 could also be a potential therapeutic agent to maintain the stability of the BRB in ischemic retinopathy. |
Databáze: | OpenAIRE |
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