SWI/SNF chromatin remodeling complex is required for initiation of sex-dependent differentiation in mouse germline
| Autor: | Toshiaki Ito, Atsuki Osada, Masami Ohta, Kana Yokota, Akira Nishiyama, Yuichi Niikura, Tomohiko Tamura, Yoichi Sekita, Tohru Kimura |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
endocrine system DNA Repair Chromosomal Proteins Non-Histone Germline development Science Mitosis Article Mice Oogenesis Sex Factors Animals Mice Knockout Multidisciplinary urogenital system Gene Expression Profiling fungi Computational Biology Cell Differentiation Chromatin Assembly and Disassembly Meiosis Gene Ontology Germ Cells Multiprotein Complexes Differentiation Oocytes Medicine Female DNA Damage |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-13 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Sexual reproduction involves the creation of sex-dependent gametes, oocytes and sperm. In mammals, sexually dimorphic differentiation commences in the primordial germ cells (PGCs) in embryonic gonads; PGCs in ovaries and testes differentiate into meiotic primary oocytes and mitotically quiescent prospermatogonia, respectively. Here, we show that the transition from PGCs to sex-specific germ cells was abrogated in conditional knockout mice carrying a null mutation of Smarcb1 (also known as Snf5) gene, which encodes a core subunit of the SWI/SNF chromatin remodeling complex. In female mutant mice, failure to upregulate meiosis-related genes resulted in impaired meiotic entry and progression, including defects in synapsis formation and DNA double strand break repair. Mutant male mice exhibited delayed mitotic arrest and DNA hypomethylation in retrotransposons and imprinted genes, resulting from aberrant expression of genes related to growth and de novo DNA methylation. Collectively, our results demonstrate that the SWI/SNF complex is required for transcriptional reprogramming in the initiation of sex-dependent differentiation of germ cells. |
| Databáze: | OpenAIRE |
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