Analysis of laminar activity in normal and injured rat spinal cord by manganese enhanced MRI
| Autor: | Didier Orsal, Olivier Stettler, Jean-Pierre Renou, Philippe Mailly, Jean-Marie Bonny, Abdelatif BenMoussa |
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| Rok vydání: | 2007 |
| Předmět: |
Cognitive Neuroscience
Contrast Media Chlorides medicine Image Processing Computer-Assisted Animals Manganese enhanced mri Longitudinal Studies Rats Wistar Spinal cord injury Spinal Cord Injuries medicine.diagnostic_test Chemistry Magnetic resonance imaging medicine.disease Spinal cord Magnetic Resonance Imaging Rats Behavioral analysis medicine.anatomical_structure Neurology Manganese Compounds Female Neuroscience Ex vivo Biomedical engineering |
| Zdroj: | NeuroImage. 40(4) |
| ISSN: | 1053-8119 |
| Popis: | The present study provides an account of a sensitive and rapid experimental approach for MRI visualization and analysis of spinal cord (SC) laminar activity in normal and injured animals. This approach is based upon neuronal activity-dependant manganese (Mn) uptake after focal SC injection of MnCl 2 , and subsequent ex-vivo magnetic resonance imaging (MRI) of activated SC pathways. The method was designed as an alternative to time-intensive histochemical and behavioral approaches typically used for analysis of spinal cord injury (SCI) and our results provide both anatomical and functional insights. We show that ex vivo imaging can determine layer-specific activity over an extended region of the rat SC. In addition, we demonstrate that the Mn concentration profile along the SC axis accurately reflects the type of SC injury. The approach is flexible since MRI analysis can be done immediately after animal sacrifice, or alternatively several days later, without a loss of sensitivity. Moreover, the integrity and functional state of SC circuitry can be analyzed in less than 1 h whereas several days and weeks are necessary to perform classical histochemical and behavioral analysis. Thus our method can be used for precise assessment of the extent of dysfunction or change in SC disorders and may facilitate the screening of molecules with therapeutic potential after SC injury. |
| Databáze: | OpenAIRE |
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