Uptake of 5-hydroxytryptamine in rat isolated atria
| Autor: | Y. Cohen, Françoise Beslot, M. Davy, Charles El Rawadi, M. Heimburger, Michèle Midol-Monnet |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
medicine.medical_specialty Serotonin Sympathetic Nervous System In Vitro Techniques Rats Sprague-Dawley chemistry.chemical_compound Norepinephrine Internal medicine Desipramine medicine Animals Heart Atria Incubation Chromatography High Pressure Liquid Pharmacology Atrium (architecture) Myocardium Heart Reserpine Hydroxyindoleacetic Acid Rats Endocrinology Mechanism of action chemistry Indalpine medicine.symptom Intracellular medicine.drug |
| Zdroj: | General pharmacology. 23(4) |
| ISSN: | 0306-3623 |
| Popis: | 1. 1. The mode of 5-hydroxytryptamine (5-HT) uptake by the rat isolated atria was studied and compared to noradrenaline (NA) uptake. 2. 2. Rat isolated atria were incubated with 14 C-5-HT (46 μM) or 3 H-NA (0.4 μM). After washing, the radioactivity fixed in atria was counted and the total NA, 5-HT and 5-hydroxyindol-3-acetic acid (5-HIAA) atria contents were measured by HPLC. 3. 3. 14 C-5-HT uptake was reduced in atria from 6-hydroxydopamine (100 mg/kg, i.p., 48 hr before experiments) or reserpine (2.5 mg/kg, i.p., 24 and 48 hr before experiments) pretreated rats. 4. 4. The incubation of atria with 5-HT (50 μM) at the same time as 3 H-NA reduced the 3 H-NA value fixed. 5. 5. Addition of desipramine (1 μM) or hydrocortisone (150 μM) before the incubation of atria with 14 C-5-HT was without any effect on 14 C-5-HT uptake. In contrast, fluvoxamine (1 μM) or indalpine (5 μM) caused a slight inhibition. 6. 6. These data indicate that 5-HT is taken into the NA storage vesicles within the atria sympathetic nerves. This uptake does not use the NA carrier and involves partly the 5-HT carrier. An extraneuronal accumulation was noticed and a part of it is intracellular. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|