The receptor tyrosine phosphatase CRYPalpha promotes intraretinal axon growth
| Autor: | Fawaz G. Haj, Andrew W. Stoker, John L. Bixby, Matthias M. Ledig, Bernhard K. Mueller |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Retinal Ganglion Cells
actin cytoskeleton integrin Receptor-Like Protein Tyrosine Phosphatases Protein tyrosine phosphatase Cell Communication Ligands Avian Proteins 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Laminin axon growth medicine Animals Axon Growth cone receptor protein tyrosine phosphatase Cells Cultured 030304 developmental biology 0303 health sciences biology Retinal Cell Biology Actin cytoskeleton Axons Cell biology growth cone medicine.anatomical_structure Retinal ganglion cell chemistry biology.protein Original Article sense organs Protein Tyrosine Phosphatases Cell Adhesion Molecules 030217 neurology & neurosurgery Signal Transduction |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | Retinal ganglion cell axons grow towards the optic fissure in close contact with the basal membrane, an excellent growth substratum. One of the ligands of receptor tyrosine phosphatase CRYPα is located on the retinal and tectal basal membranes. To analyze the role of this RPTP and its ligand in intraretinal growth and guidance of ganglion cell axons, we disrupted ligand- receptor interactions on the retinal basal membrane in culture. Antibodies against CRYPα strongly reduced retinal axon growth on the basal membrane, and induced a dramatic change in morphology of retinal growth cones, reducing the size of growth cone lamellipodia. A similar effect was observed by blocking the ligand with a CRYPα ectodomain fusion protein. These effects did not occur, or were much reduced, when axons were grown either on laminin-1, on matrigel or on basal membranes with glial endfeet removed. This indicates that a ligand for CRYPα is located on glial endfeet. These results show for the first time in vertebrates that the interaction of a receptor tyrosine phosphatase with its ligand is crucial not only for promotion of retinal axon growth but also for maintenance of retinal growth cone lamellipodia on basal membranes. |
| Databáze: | OpenAIRE |
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