High performance liquid chromatography determination of dexamethasone in plasma to evaluate its systemic absorption following intra-space pterygomandibular injection of twin-mix (mixture of 2 % lignocaine with 1:200,000 epinephrine and 4 mg dexamethasone): randomized control trial
| Autor: | Ashwini Deshpande, Sanjeev Kumar Sahu, Yogesh Sharma, Darpan Bhargava, Ankit Pandey, Shaji Thomas, Suyash Dubey, K. Sreekumar, Piush Khare |
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| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Epinephrine Visual Analog Scale Lidocaine medicine.drug_class Anesthesia Dental Mandible Oral Mucosal Absorption Dexamethasone Injections 03 medical and health sciences 0302 clinical medicine Double-Blind Method medicine Humans Local anesthesia Prospective Studies 030223 otorhinolaryngology Chromatography High Pressure Liquid business.industry Local anesthetic 030206 dentistry Venous blood Pterygomandibular space Drug Combinations Otorhinolaryngology Anesthesia Tooth Extraction Female Molar Third Surgery Oral Surgery Intramuscular injection business Anesthesia Local medicine.drug |
| Zdroj: | Oral and Maxillofacial Surgery. 20:259-264 |
| ISSN: | 1865-1569 1865-1550 |
| DOI: | 10.1007/s10006-016-0564-3 |
| Popis: | To determine systemic absorption of dexamethasone by detection of plasma concentration using high performance liquid chromatography following its administration along with local anesthetic agent as a mixture via pterygomandibular space. A prospective randomized double-blind clinical study was undertaken to analyze the plasma concentration of dexamethasone after intra-space pterygomandibular injection along with local anesthesia. The study was performed as per split mouth model where the mandibular quadrant allocation was done on a random basis considering each of the 30 patients is included in the two study interventions (SS and CS). For the study site (SS) procedures, dexamethasone was administered as a mixture (2 % lignocaine with 1:200,000 epinephrine and 4 mg dexamethasone) intra-space. In the control site (CS) procedures, a regular standard inferior alveolar nerve block was administered, and dexamethasone was given as intramuscular injection. The plasma dexamethasone determination was done in venous blood 30- and 60-min post injection using high performance liquid chromatography (HPLC). The clinical parameters like pain; swelling; and mouth opening on the first, third, and seventh post-operative day were analyzed and compared. No significant difference was found in the clinical parameters assessed; comparative evaluation showed less swelling in the SS interventions. The plasma concentration of dexamethasone for the CS interventions was 226 ± 47 ng/ml at 30-min and 316 ± 81.6 ng/ml at 60-min post injection, and for SS, it was 221 ± 81.6 ng/ml at 30-min and 340 ± 105 ng/ml at 60-min post injection. On inter-site (CS and SS) comparison, no statistically significant difference was ascertained in dexamethasone plasma concentration at 30-min post injection (P = 0.77) and at 60-min post injection. (P = 0.32). Intra-space (pterygomandibular space) administration of dexamethasone can achieve statistically similar plasma concentration of the drug as when the same dose is administered intramuscularly with demonstration of similar clinical effects. |
| Databáze: | OpenAIRE |
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