Periodontal bacteria aggravate experimental autoimmune myocarditis in mice
| Autor: | Jun-ichi Suzuki, Yuichi Izumi, Yuko Tada, Masahito Ogawa, Asuka Sekinishi, Norio Aoyama, Ryo Watanabe, Mitsuaki Isobe, Naho Kobayashi, Yasunobu Hirata, Tomoya Hanatani, Chisato Takamura, Ryozo Nagai, Kouji Wakayama, Norihiko Ashigaki, Hirofumi Zempo |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Autoimmune myocarditis Myocarditis Physiology Disease Myosins Body weight Lung pathology Periodontal bacteria Autoimmune Diseases Mice Risk Factors Seroepidemiologic Studies Physiology (medical) Prevalence medicine Animals Periodontitis Lung Autoantibodies Mice Inbred BALB C CD11b Antigen biology business.industry Body Weight Organ Size medicine.disease Antibodies Bacterial Disease Models Animal Matrix Metalloproteinase 9 Immunology biology.protein Cytokines Antibody Cardiology and Cardiovascular Medicine business Porphyromonas gingivalis |
| Zdroj: | American Journal of Physiology-Heart and Circulatory Physiology. 304:H740-H748 |
| ISSN: | 1522-1539 0363-6135 |
| Popis: | Periodontitis is one of the most common infections in humans. Recently, published reports assert that periodontitis is associated with cardiovascular disease. Although it is said that viral, bacterial infections and autoimmune diseases may be the cause of myocarditis, the pathogenesis of it remains unclear. The aim of this study was to investigate the influence of a periodontal pathogen on experimental autoimmune myocarditis (EAM). Porphyromonas gingivalis (P.g.), PBS as a control, were injected into the mice. Histopathological and immunohistochemical analyses were performed. We examined heart mRNA levels using quantitative RT-PCR. The anti-P.g. IgG antibody level in plasma samples of the P.g.-injected group significantly increased compared with the PBS-injected group. Histopathological analysis detected that the myocarditis-affected areas and the fibrotic area in the P.g.-injected EAM group significantly increased compared with the PBS-injected EAM group ( P < 0.05). Immunohistochemical analysis detected that more CD11b-positive cells were shown in the heart of the P.g.-injected EAM group compared with the PBS EAM-injected group ( P < 0.05). Hearts from the P.g.-injected EAM group showed significantly increased expression of monocyte chemoattractant protein-1, IFN-γ, and matrix metalloproteinase-9 (MMP-9) mRNA compared with the hearts from the PBS-injected EAM group ( P < 0.05). On day 7, serum levels of IL-6 were significantly enhanced in the P.g.-injected EAM group compared with the PBS-injected EAM group ( P < 0.05). These results showed that P.g. injection could deteriorate EAM in mice through CD11b-positive cells, cytokines, and MMP-9 expression. |
| Databáze: | OpenAIRE |
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