Detection of human telomerase RNA in the tumour-surrounding mucosa of bladder carcinomas as a marker for premalignant transformation
| Autor: | Sabine Riethdorf, Thomas Löning, Andreas Erbersdobler, Martin G. Friedrich, Lutz Riethdorf, Hartwig Huland, Sven Leuenroth |
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| Rok vydání: | 2005 |
| Předmět: |
Nephrology
In situ Adult Male medicine.medical_specialty Pathology Telomerase Urology Urinary Bladder In situ hybridization Internal medicine medicine Biomarkers Tumor Humans In Situ Hybridization Aged Carcinoma Transitional Cell Bladder cancer Chi-Square Distribution Mucous Membrane business.industry RNA Riboprobe Middle Aged medicine.disease Transitional cell carcinoma Urinary Bladder Neoplasms Case-Control Studies Neoplasm Recurrence Local business Carcinoma in Situ |
| Zdroj: | BJU international. 96(4) |
| ISSN: | 1464-4096 |
| Popis: | OBJECTIVES To investigate tumour tissue and non-malignant tumour-surrounding bladder mucosa (NTSBM) for expression of human telomerase RNA (hTR) as a possible marker for premalignant transformation of urothelial cells. MATERIAL AND METHODS From 67 patients with superficial transitional cell carcinoma (TCC), sections with representative tumour tissue and NTSBM were selected for evaluation. Sections with moderate or severe dysplasia were omitted from evaluation. hTR expression was detected with in situ hybridization using a 35S-UTP-labelled riboprobe, and analysed semiquantitatively by counting the hybridization signals. RESULTS In 45 of the 67 patients hTR expression was moderate or strong in tumour tissue, and in 20 hTR expression was moderate or strong in NTSBM. Moderate or strong hTR expression was detected in the NTSBM from 19 of 60 patients with pTa/pT1 tumours. Of the 56 patients who were treated conservatively, eight had tumour recurrence, of whom five had moderate or strong hTR expression in the TSBM, compared with only 14 of 48 patients without tumour recurrence. CONCLUSION Detecting hTR expression and the morphological distribution of hTR hybridization signals in NTSBM by in situ hybridization might indicate premalignant alterations involved in tumour recurrence. |
| Databáze: | OpenAIRE |
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