Isolation and characterization of an aspartic proteinase gene from cowpea (Vigna unguiculata L. Walp.)
| Autor: | Yasmine Zuily Fodil, Agnès d'Arcy-Lameta, Monique Gareil, Maria Helena Catelli de Carvalho, Anh Thu Pham-Thi |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Physiology
Sequence analysis Molecular Sequence Data Plant Science Genes Plant Vigna Exon Aspartic Acid Endopeptidases Genomic library Amino Acid Sequence Northern blot Cloning Molecular Gene Plant Proteins chemistry.chemical_classification Genomic Library Base Sequence biology Intron food and beverages Fabaceae biology.organism_classification Molecular biology Recombinant Proteins Enzyme chemistry Agronomy and Crop Science |
| Zdroj: | Journal of Plant Physiology. 161:971-976 |
| ISSN: | 0176-1617 |
| DOI: | 10.1016/j.jplph.2003.12.007 |
| Popis: | A cowpea (Vigna unguiculata cv. EPACE-1) aspartic proteinase (AP) gene was isolated by genomic Library screening. Sequence analysis shows that this AP gene follows the same pattern of intron/exon number and organization as the other isolated plant AP genes, which are distinct from other solved AP genes. Northern blot analysis revealed that cowpea AP accumulates in leaves and stems but not in roots, indicating tissue-specific expression. An increased accumulation of transcripts during senescence suggests enzyme involvement in this process. |
| Databáze: | OpenAIRE |
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