Impact of cell adhesion and migration on nanoparticle uptake and cellular toxicity
| Autor: | Mukund Bahadur Koirala, Santosh Aryal, Arunkumar Pitchaimani, Tuyen Duong Thanh Nguyen, Yuntao Zhang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Cell Survival
Surface Properties Stereochemistry Cell Metal Nanoparticles 02 engineering and technology 010402 general chemistry Toxicology 01 natural sciences Mice Cell Movement Cell Line Tumor Cell Adhesion medicine Animals Humans Cytotoxic T cell Cell adhesion Cytotoxicity Chemistry Biological Transport Cell migration General Medicine 021001 nanoscience & nanotechnology 0104 chemical sciences medicine.anatomical_structure Colloidal gold Cell culture Cancer cell Biophysics Gold 0210 nano-technology |
| Zdroj: | Toxicology in Vitro. 43:29-39 |
| ISSN: | 0887-2333 |
| DOI: | 10.1016/j.tiv.2017.05.020 |
| Popis: | In vitro cell-nanoparticle (NP) studies involve exposure of NPs onto the monolayer cells growing at the bottom of a culture plate, and assumed that the NPs evenly distributed for a dose-responsive effect. However, only a few proportion of the administered dose reaches the cells depending on their size, shape, surface, and density. Often the amount incubated (administered dose) is misled as a responsive dose. Herein, we proposed a cell adhesion-migration (CAM) strategy, where cells incubated with the NP coated cell culture substrate to maximize the cell-NP interaction and investigated the physiological properties of the cells. In the present study, cell adhesion and migration pattern of human breast cancer cell (MCF-7) and mouse melanoma cell (B16-F10) on cell culture substrate decorated with toxic (cetyltrimethylammonium bromide, CTAB) and biocompatible (poly (sodium 4-styrenesulphonate), PSS) gold nanoparticles (AuNPs) of different sizes (5 and 40nm) were investigated and evaluated for cellular uptake efficiency, proliferation, and toxicity. Results showed enhanced cell adhesion, migration, and nanoparticle uptake only on biocompatible PSS coated AuNP, irrespective of its size. Whereas, cytotoxic NP shows retard proliferation with reduced cellular uptake efficiency. Considering the importance of cell adhesion and migration on cellular uptake and cytotoxicity assessment of nanoparticle, CAM strategy would hold great promises in cell-NP interaction studies. |
| Databáze: | OpenAIRE |
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