Methylmercury Induced Neurotoxicity and the Influence of Selenium in the Brains of Adult Zebrafish (Danio rerio)
| Autor: | Anne-Katrine Lundebye, Heidi Amlund, J.D. Rasinger, Ståle Ellingsen, Samuel Penglase |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
contaminant dietary exposure interaction mechanisms mercury methylmercury nutrient proteomics selenium 010501 environmental sciences 01 natural sciences Oxidative Phosphorylation lcsh:Chemistry chemistry.chemical_compound Water Pollutants Selenomethionine Methylmercury Zebrafish lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification biology TOR Serine-Threonine Kinases Brain Gap Junctions General Medicine Methylmercury Compounds Mitochondria Computer Science Applications Environmental chemistry medicine.medical_specialty Danio chemistry.chemical_element Oxidative phosphorylation Article Catalysis Inorganic Chemistry 03 medical and health sciences Internal medicine medicine Animals Physical and Theoretical Chemistry Molecular Biology PI3K/AKT/mTOR pathway 0105 earth and related environmental sciences Reactive oxygen species Organic Chemistry Neurotoxicity biology.organism_classification medicine.disease 030104 developmental biology Endocrinology chemistry lcsh:Biology (General) lcsh:QD1-999 Reactive Oxygen Species Selenium |
| Zdroj: | International Journal of Molecular Sciences; Volume 18; Issue 4; Pages: 725 International Journal of Molecular Sciences, Vol 18, Iss 4, p 725 (2017) 18:725 International Journal of Molecular Sciences |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms18040725 |
| Popis: | The neurotoxicity of methylmercury (MeHg) is well characterised, and the ameliorating effects of selenium have been described. However, little is known about the molecular mechanisms behind this contaminant-nutrient interaction. We investigated the influence of selenium (as selenomethionine, SeMet) and MeHg on mercury accumulation and protein expression in the brain of adult zebrafish (Danio rerio). Fish were fed diets containing elevated levels of MeHg and/or SeMet in a 2 × 2 full factorial design for eight weeks. Mercury concentrations were highest in the brain tissue of MeHg-exposed fish compared to the controls, whereas lower levels of mercury were found in the brain of zebrafish fed both MeHg and SeMet compared with the fish fed MeHg alone. The expression levels of proteins associated with gap junction signalling, oxidative phosphorylation, and mitochondrial dysfunction were significantly (p < 0.05) altered in the brain of zebrafish after exposure to MeHg and SeMet alone or in combination. Analysis of upstream regulators indicated that these changes were linked to the mammalian target of rapamycin (mTOR) pathways, which were activated by MeHg and inhibited by SeMet, possibly through a reactive oxygen species mediated differential activation of RICTOR, the rapamycin-insensitive binding partner of mTOR. |
| Databáze: | OpenAIRE |
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