New stable isotope method to measure protein digestibility and response to pancreatic enzyme intake in cystic fibrosis
| Autor: | Mariëlle P.K.J. Engelen, Paula J. Anderson, Nicolaas E. P. Deutz, Gulnur Com |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Male medicine.medical_specialty Adolescent Cystic Fibrosis Phenylalanine Critical Care and Intensive Care Medicine Cystic fibrosis Article Young Adult chemistry.chemical_compound Bacterial Proteins Internal medicine Protein digestibility Spirulina Citrulline medicine Humans Child Pancreas Radioisotopes Gastrointestinal tract Nutrition and Dietetics Dose-Response Relationship Drug Stable isotope ratio business.industry medicine.disease Healthy Volunteers Gastrointestinal Tract Muscular Atrophy Endocrinology medicine.anatomical_structure chemistry Case-Control Studies Isotope Labeling Dietary Supplements Body Composition Female Dietary Proteins Digestion business |
| Zdroj: | Clinical Nutrition. 33:1024-1032 |
| ISSN: | 0261-5614 |
| Popis: | Adequate protein intake and digestion are necessary to prevent muscle wasting in cystic fibrosis (CF). Accurate and easy-to-use methodology to quantify protein maldigestion is lacking in CF.To measure protein digestibility and the response to pancreatic enzyme intake in CF by using a new stable isotope methodology.In 19 CF and 8 healthy subjects, protein digestibility was quantified during continuous (sip) feeding for 6 h by adding (15)N-labeled spirulina protein and L-[ring-(2)H5]phenylalanine (PHE) to the nutrition and measuring plasma ratio [(15)N]PHE to [(2)H5]PHE. Pancreatic enzymes were ingested after 2 h in CF and the response in protein digestibility was assessed. To exclude difference in mucosal function, postabsorptive whole-body citrulline (CIT) production rate was measured by L-[5-(13)C-5,5-(2)H2]-CIT pulse and blood samples were taken to analyze tracer-tracee ratios.Protein digestibility was severely reduced in the CF group (47% of healthy subjects; P 0.001). Intake of pancreatic enzymes induced a slow increase in protein digestibility in CF until 90% of values obtained by healthy subjects. Maximal digestibility was reached at 100 min and maintained for 80 min. Stratification into CF children (n = 10) and adults showed comparable values for protein digestibility and similar kinetic responses to pancreatic enzyme intake. Whole-body citrulline production was elevated in CF indicating preserved mucosal function.Protein digestibility is severely compromised in patients with CF as measured by this novel and easy-to-use stable isotope approach. Pancreatic enzymes are able to normalize protein digestibility in CF, albeit with a severe delay. Registration ClinicalTrials.gov = NCT01494909. |
| Databáze: | OpenAIRE |
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