Organic solvents can influence acetylcholine neurotransmission in Caenorhabditis elegans
| Autor: | Raman Lakshmisundaram, Velmurugan Janaki Devi, Jamuna R. Subramaniam |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Aldicarb
Ethyl acetate chemistry.chemical_compound medicine Paralysis Caenorhabditis elegans Nitrogen gas Chloroform Ethanol Chromatography biology Drug discovery General Neuroscience Neurotransmission Clinical Neuroscience biology.organism_classification Acetylcholine chemistry C. elegans Esterase inhibitor Hexane Research Article medicine.drug |
| Zdroj: | Annals of Neurosciences |
| ISSN: | 0976-3260 0972-7531 |
| Popis: | Background Identification of novel drugs by bio-prospecting natural products like various parts of the plants, or other extracts and drug discovery requires differential fractionation with various organic solvents followed by their concentration through evaporation under nitrogen gas, which is a standard practice. Purpose Determination of contribution of vehicle control of organic solvents (chloroform, ethanol, ethyl acetate and n-hexane) processed in the similar manner in the modulation of acetylcholine(ACh) neurotransmission in Caenorhabditis elegans, Aldicarb induced paralysis assay. Methods The organic solvents concentrated as described in background was used to identify their contribution in ACh modulation through ACh esterase inhibitor, Aldicarb, treatment of C. elegans, which leads to time dependent paralysis of the worms. Results The vehicle, organic solvents, control itself bestows modulation of acetylcholine release as Aldicarb resistance in C. elegans. Conclusion Given the exorbitant cost and time taken for drug discovery, identification of efficacy of bioactive molecules fractionated through organic solvents and concentrated under nitrogen gas should have appropriate vehicle control as described above to avoid the rate of false positives. This is universally applicable whether the drug is chemically synthesized or purified from natural products. |
| Databáze: | OpenAIRE |
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