Analysis of the immune response of Hantaan virus nucleocapsid protein-specific CD8+ T cells in mice
| Autor: | Koichi Araki, Ichiro Nakamura, Jiro Arikawa, Kumiko Yoshimatsu, Kiichi Kajino, Midori Taruishi, Megumi Okumura |
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| Rok vydání: | 2006 |
| Předmět: |
Epitopes
T-Lymphocyte Biology Persistent infection CD8-Positive T-Lymphocytes Major histocompatibility complex Epitope Interleukin 21 Interferon-gamma Mice Immune system Antigen T-Lymphocyte Subsets Virology Cytotoxic T cell Animals Hantavirus-specific CD8+ T cell Mice Inbred BALB C Viral Core Proteins T lymphocyte Molecular biology Hantaan virus Hemorrhagic Fever with Renal Syndrome biology.protein MHC tetramer Capsid Proteins Female CD8 Epitope Mapping Hantavirus |
| Zdroj: | Virology. 365(2) |
| ISSN: | 0042-6822 |
| Popis: | The major histocompatibility complex (MHC) class-I restricted epitope of Hantaan virus nucleocapsid protein (N) was identified using overlapping peptides and BALB/c mice. Using the MHC tetramer derived from the epitope, we found that the level of N-specific CD8(+) T cells increased to approximately 20% of all antigen-specific CD8(+) T cells in a mouse model of transient infection. However, N-specific CD8(+) T cells were undetectable in a mouse model of persistent infection, both in the persistently infected phase and in the convalescent phase. Levels of CD8(+) T cells producing interferon-gamma were weak in both the acute and convalescent phases in the persistently infected model. These results indicate that hantavirus strongly suppresses the production of N-specific CD8(+) T cells throughout the course of infection in persistently infected mice. Moreover, N-specific CD8(+) T cells were not effective in recovering persistently infected mice, despite the existence of abundant N antigen in vivo. |
| Databáze: | OpenAIRE |
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