Selective detection of endogenous H2S in living cells and the mouse hippocampus using a ratiometric fluorescent probe
| Autor: | Jing Zhao, Yi Liu, Ling Zhang, Wen-qi Meng, Yunsheng Xue, Fang Zou, Liang Lu, Cheng Li |
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| Rok vydání: | 2014 |
| Předmět: |
Cystathionine beta-Synthase
Down-Regulation Gene Expression Endogeny Hippocampus Article Mice Limit of Detection Gene expression Animals Humans Chronic stress Hydrogen Sulfide RNA Messenger Bovine serum albumin Gasotransmitters Fluorescent Dyes Multidisciplinary biology Depression Chemistry Fluorescence Cystathionine beta synthase Disease Models Animal Naphthalimides Spectrometry Fluorescence Molecular Probes MCF-7 Cells biology.protein Biophysics Cattle Carbamates Signal transduction Stress Psychological Signal Transduction |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep05870 |
| Popis: | As one of three gasotransmitters, the fundamental signalling roles of hydrogen sulphide are receiving increasing attention. New tools for the accurate detection of hydrogen sulphide in cells and tissues are in demand to probe its biological functions. We report the p-nitrobenzyl-based ratiometric fluorescent probe RHP-2, which features a low detection limit, high selectivity and good photostability. The emission intensity ratios had a good linear relationship with the sulphide concentrations in PBS buffer and bovine serum. Our probe was applied to the ratiometric determination and imaging of endogenous H2S in living cells. Furthermore, RHP-2 was used as an effective tool to measure endogenous H2S in the mouse hippocampus. We observed a significant reduction in sulphide concentrations and downregulated expression of cystathionine β-synthetase (CBS) mRNA and CBS protein in the mouse hippocampus in a chronic unpredictable mild stress (CUMS)-induced depression model. These data suggested that decreased concentrations of endogenous H2S may be involved in the pathogenesis of chronic stress depression. |
| Databáze: | OpenAIRE |
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