Prevalence and Cellular Reservoir of Latent Human Herpesvirus 6 in Tonsillar Lymphoid Tissue
| Autor: | Rana Domiati-Saad, Karen S. Roush, D. Brian Dawson, Linda R. Margraf, Beverly Barton Rogers, Karen Krisher, Richard H. Scheuermann |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Pathology medicine.medical_specialty Adolescent Herpesvirus 6 Human viruses Palatine Tonsil Enzyme-Linked Immunosorbent Assay In situ hybridization medicine.disease_cause Polymerase Chain Reaction Herpesviridae stomatognathic system Tonsillar crypts Exanthema Subitum medicine Humans Lymphocytes Child In Situ Hybridization DNA Primers biology medicine.diagnostic_test Infant virus diseases General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Lymphatic system medicine.anatomical_structure Child Preschool Tonsil DNA Viral Tonsillar Lymphoid Tissue Immunology Female Human herpesvirus 6 Fluorescence in situ hybridization |
| Zdroj: | American Journal of Clinical Pathology. 116:648-654 |
| ISSN: | 1943-7722 0002-9173 |
| Popis: | There are few studies that examine prevalence, quantity, and cellular proclivity of latent human herpesvirus 6 (HHV-6) in healthy populations. We examined 69 tonsils with paired blood specimens from children without evidence of acute infection. By polymerase chain reaction (PCR), HHV-6 was detected at low levels in 100% of tonsils and 39% of blood samples (n = 27), suggesting that prevalence of latent HHV-6 infection is high in children and may be underestimated by PCR analysis of blood. Although HHV-6A and HHV-6B were detected, HHV-6B predominated, being found in 97% of samples (n = 67). Tonsil sections from 7 cases were examined by in situ hybridization using 2 HHV-6 probes and immunohistochemical analysis. Using both in situ hybridization and immunohistochemical analysis, all tissues revealed marked HHV-6-specific staining in the squamous epithelium of the tonsillar crypts and rare positive lymphocytes. We conclude that HHV-6 is present universally in tonsils of children, and tonsillar epithelium may be an important viral reservoir in latent infection. |
| Databáze: | OpenAIRE |
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