Peanut components measured by ISAC
| Autor: | R. Gerth van Wijk, N. J. T. Arends, Marco W.J. Schreurs, H. K. Brand, H. de Groot, J. A. M. Emons |
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| Přispěvatelé: | Pediatric surgery, Pathology, Immunology, Pediatrics, Internal Medicine |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Allergy
Peanut allergy Immunology Component resolved diagnostics medicine.disease_cause Immunoglobulin E Allergen Ara h 6 medicine Ara h 2 Immunology and Allergy Clinical significance Multiplex Food science Molecular Biology Children ISAC biology business.industry Research fungi Multiplex analysis food and beverages Food challenge test RC581-607 biochemical phenomena metabolism and nutrition medicine.disease carbohydrates (lipids) biology.protein lipids (amino acids peptides and proteins) Immunologic diseases. Allergy business |
| Zdroj: | Brand, H K, Schreurs, M W J, Emons, J A M, Gerth van Wijk, R, de Groot, H & Arends, N J T 2021, ' Peanut components measured by ISAC : comparison with ImmunoCap and clinical relevance in peanut allergic children ', Clinical and molecular allergy : CMA, vol. 19, no. 1, 14, pp. 14 . https://doi.org/10.1186/s12948-021-00153-w Clinical and Molecular Allergy : CMA Clinical and molecular allergy : CMA, 19(1):14. BioMed Central Ltd. Clinical and Molecular Allergy, Vol 19, Iss 1, Pp 1-11 (2021) Clinical and Molecular Allergy, 19(1):14. BioMed Central Ltd. |
| ISSN: | 1476-7961 |
| Popis: | Background Specific IgE (sIgE) against the peanut component Arachis hypogaea (Ara h) 2 has been shown to be the most important allergen to discriminate between peanut allergy and peanut tolerance. Several studies determined sIgE cut off values for Ara h 2, determined by singleplex measurements. However, cut off values for Ara h 2 from multiplex arrays are less well defined. The aim of this study was to evaluate the correlation between Ara h 2 sIgE determined by singleplex versus multiplex measurements and to assess the diagnostic value of the different peanut components included in Immuno Solid-phase Allergen Chip (ISAC) multiplex analysis in children with a suspected peanut allergy. Methods In this retrospective study we analyzed Ara h 2 sIgE values with singleplex Fluorescence Enzyme Immunoassay (FEIA, ImmunoCap) and multiplex microarray (ISAC) measurements in 117 children with a suspected peanut allergy. Also, other peanut components measured by ISAC were analyzed. Double blinded placebo controlled oral food challenges were used as golden standard. Results Among all studied peanut components FEIA Ara h 2 sIgE showed the highest area under the curve (AUC, 0.922), followed by ISAC Ara h 6 and Ara h 2 sIgE with AUCs of respectively 0.906 and 0.902. Best cut off values to diagnose peanut allergy were 4.40 kU/l for FEIA Ara h 2 sIgE and, 7.43 ISU and 8.13 ISU for respectively Ara h 2 and Ara h 6 sIgE in ISAC microarray. Ara h 2 sIgE determined in FEIA and ISAC showed a good correlation (r = 0.88; p Conclusion Ara h 6 and Ara h 2 sIgE in multiplex ISAC are both good predictors of clinical peanut allergy in Dutch children, and their performance is comparable to the use of Ara h 2 in singleplex FEIA. The simultaneous measurement of different peanut components using ISAC is an advantage and clinically useful to detect peanut allergic children that are Ara h 2 negative but sensitized to other peanut proteins such as Ara h 6. |
| Databáze: | OpenAIRE |
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