Role of Free Radicals in the Mechanism of The Hydrazine- Induced Formation of Megamitochondria
| Autor: | Takashi Wakabayashi, Xinran Liu, Michal Wozniak, Mariusz Karbowski, Tatsuo Matsuhashi, Jedrzej Antosiewicz |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Fatty Acid Desaturases
Male Xanthine Oxidase Antioxidant Free Radicals medicine.drug_class Allopurinol medicine.medical_treatment Mitochondria Liver In Vitro Techniques medicine.disease_cause Biochemistry Cyclic N-Oxides Lipid peroxidation chemistry.chemical_compound Oxygen Consumption Physiology (medical) medicine Animals Hydrazine (antidepressant) Enzyme Inhibitors Rats Wistar Xanthine oxidase Xanthine oxidase inhibitor Chemistry Megamitochondria Free Radical Scavengers Rats Microscopy Electron Oxidative Stress Hydrazines Purines Spin Labels Lipid Peroxidation Reactive Oxygen Species Oxidative stress medicine.drug |
| Zdroj: | Free Radical Biology and Medicine. 23:285-293 |
| ISSN: | 0891-5849 |
| Popis: | The effect of 4-hydroxy-2,2,6,6-tetramethyl-piperidine-1-oxyl(4-OH-TEMPO), a scavenger for free radicals, and 4-hydroxypyrazolo[3,4-d(pyrimidine)allopurinol], a xanthine oxidase inhibitor, on the hydrazine-induced changes of mitochondrial ultrastructure and those in the antioxidant system of the liver were investigated using rats as experimental animals. Animals were placed on a powdered diet containing 0.5% hydrazine for 7 d in the presence and absence of a combined treatment with 4-OH-TEMPO or allopurinol. Results obtained were as follows. 4-OH- TEMPO completely prevented the hydrazine-induced formation of megamitochondria in the liver, while it was partly prevented by allopurinol. The following changes observed in hydrazine-treated animals were improved almost completely by 4-OH-TEMPO:decreases in the body weight and liver weight; lowered rates of ADP-stimulated respiration and coupling efficiency of hepatic mitochondria; remarkable elevation of the level of lipid peroxidation. Improving effects of allopurinol were incomplete. The present results suggest that free radicals may play a key role in the mechanism of the hydrazine-induced formation of megamitochondria and that a part of free radicals generated during the hydrazine intoxication is ascribed to the degradation of purine nucleotides via xanthine oxidase. A general mechanism of the megamitochondria formation induced in various pathologic conditions besides the case of hydrazine are discussed. |
| Databáze: | OpenAIRE |
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