Implication of Membrane Androgen Receptor (ZIP9) in Cell Senescence in Regressed Testes of the Bank Vole
| Autor: | Malgorzata Kotula-Balak, Anna Korzekwa, Ewelina Gorowska-Wojtowicz, Anna Hejmej, Magdalena Profaska-Szymik, Ryszard Tuz, Piotr Pawlicki, Kazimierz Tarasiuk, Anna Galuszka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Senescence
Male senescence medicine.drug_class testes androgen Biology Catalysis Article Inorganic Chemistry Andrology lcsh:Chemistry medicine Cyclic AMP Animals Physical and Theoretical Chemistry bank vole Molecular Biology lcsh:QH301-705.5 Spectroscopy Testosterone Cellular Senescence Mitogen-Activated Protein Kinase 3 Kinase Arvicolinae Organic Chemistry Epithelial Cells General Medicine Seminiferous Tubules Androgen Sertoli cell Epithelium Computer Science Applications medicine.anatomical_structure Seminiferous tubule lcsh:Biology (General) lcsh:QD1-999 Receptors Androgen Membrane androgen receptor |
| Zdroj: | International Journal of Molecular Sciences Volume 21 Issue 18 International Journal of Molecular Sciences, Vol 21, Iss 6888, p 6888 (2020) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms21186888 |
| Popis: | Here, we studied the impact of exposure to short daylight conditions on the expression of senescence marker (p16), membrane androgen receptor (ZIP9) and extracellular signal-regulated kinase (ERK 1/2), as well as cyclic AMP (cAMP) and testosterone levels in the testes of mature bank voles. Animals were assigned to groups based on an analysis of testis diameter, weight, seminiferous tubule diameter and the interstitial tissue area: group 1, not fully regressed (the highest parameters) group 2 (medium parameters) or group 3, regressed (the lowest parameters). Cells positive for p16 were observed only in the seminiferous tubule epithelium. However, in groups 1 and 2, these were mostly cells sloughed into the tubule lumen. In group 3, senescent cells resided in between cells of the seminiferous epithelium. Staining for ZIP9 was found in Sertoli cells. Western blot analysis showed a trend towards a decreased expression of p16 and ZIP9 in the testes of the voles in groups 2 and 3, compared to group 1. In addition, a trend towards an increased expression of ERK, as well as an increase of cAMP and testosterone levels, was revealed in group 2. In the regressed testes, a functional link exists between senescence and androgen levels with implication of ZIP9 and cAMP/ERK signaling pathways. |
| Databáze: | OpenAIRE |
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