Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes
| Autor: | Maria-Luisa Fernández-Cruz, Luis Alte, Mona Connolly, Helmut Segner, Alba Quesada-García, José María Navas |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
primary hepatocyte
Silver Health Toxicology and Mutagenesis lcsh:Medicine rainbow trout (Oncorhynchus mykiss) Biology Silver nanoparticle Article in vitro cell lines Cell Line Toxicology Animals 14. Life underwater Cytotoxicity Primary (chemistry) 630 Agriculture Liver cell lcsh:R Public Health Environmental and Occupational Health In vitro AgNPs Biochemistry Cell culture Oncorhynchus mykiss Toxicity Hepatocytes cytotoxicity Nanoparticles Rainbow trout |
| Zdroj: | Connolly, Mona; Fernandez-Cruz, Maria-Luisa; Quesada-Garcia, Alba; Alte, Luis; Segner, Helmut; Navas, Jose M (2015). Comparative Cytotoxicity Study of Silver Nanoparticles (AgNPs) in a Variety of Rainbow Trout Cell Lines (RTL-W1, RTH-149, RTG-2) and Primary Hepatocytes. International journal of environmental research and public health, 12(5), pp. 5386-5405. Molecular Diversity Preservation International MDPI 10.3390/ijerph120505386 International Journal of Environmental Research and Public Health International Journal of Environmental Research and Public Health, Vol 12, Iss 5, Pp 5386-5405 (2015) Repositorio de Resultados de Investigación del INIA INIA: Repositorio de Resultados de Investigación del INIA Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA Volume 12 Issue 5 Pages 5386-5405 |
| DOI: | 10.3390/ijerph120505386 |
| Popis: | Among all classes of nanomaterials, silver nanoparticles (AgNPs) have potentially an important ecotoxicological impact, especially in freshwater environments. Fish are particularly susceptible to the toxic effects of silver ions and, with knowledge gaps regarding the contribution of dissolution and unique particle effects to AgNP toxicity, they represent a group of vulnerable organisms. Using cell lines (RTL-W1, RTH-149, RTG-2) and primary hepatocytes of rainbow trout (Oncorhynchus mykiss) as in vitro test systems, we assessed the cytotoxicity of the representative AgNP, NM-300K, and AgNO3 as an Ag+ ion source. Lack of AgNP interference with the cytotoxicity assays (AlamarBlue, CFDA-AM, NRU assay) and their simultaneous application point to the compatibility and usefulness of such a battery of assays. The RTH-149 and RTL-W1 liver cell lines exhibited similar sensitivity as primary hepatocytes towards AgNP toxicity. Leibovitz’s L-15 culture medium composition (high amino acid content) had an important influence on the behaviour and toxicity of AgNPs towards the RTL-W1 cell line. The obtained results demonstrate that, with careful consideration, such an in vitro approach can provide valuable toxicological data to be used in an integrated testing strategy for NM-300K risk assessment. © 2015 by the authors; licensee MDPI, Basel, Switzerland. |
| Databáze: | OpenAIRE |
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