Association between circulating interleukin-1 beta (IL-1β) levels and IL-1β C–511T polymorphism with cervical cancer risk in Egyptian women
| Autor: | Manal M. El Behery, Magdy A. Al-Tahhan, Rasha L. Etewa |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
medicine.medical_specialty Pathology Genotype Clinical chemistry Interleukin-1beta Clinical Biochemistry Uterine Cervical Neoplasms Single-nucleotide polymorphism Biology Polymorphism Single Nucleotide Risk Assessment Gastroenterology Young Adult Gene Frequency Risk Factors Internal medicine Odds Ratio medicine Humans Allele Molecular Biology Cervix Aged Cervical cancer Promoter Cell Biology General Medicine Plasma levels Middle Aged medicine.disease Logistic Models medicine.anatomical_structure Case-Control Studies Egypt Female |
| Zdroj: | Molecular and Cellular Biochemistry. 353:159-165 |
| ISSN: | 1573-4919 0300-8177 |
| Popis: | Cancer cervix is one of the leading causes of cancer-related mortality among women worldwide. It is believed that the host genetic factors such as inflammation-induced cytokines may play a role in cervical carcinogenesis. The interleukin-1β (IL-1β) gene contains several single nucleotide polymorphisms. One of them, C-511T, which in the promoter region has been associated with increased IL-1β production and with increased risk of developing cancers. We assessed the association between the IL-1β C-511T polymorphism and cervical cancer risk in a case-control study among 100 histopathologically confirmed Egyptian women with cervical cancer and 50 age-matched, cervical cytology negative, healthy controls by polymerase chain reaction-restriction fragment length polymorphism. Plasma levels of IL-1β were assayed by enzyme-linked immunosorbent assay. There was significant increase in the mean plasma IL-1β level in cervical cancer cases (43.40 ± 25.95 pg/ml) when compared with controls (30.51 ± 18.28 pg/ml, P = 0.002). The plasma levels above the 75th percentile of controls (IL-1β ≥ 45.74 pg/ml) were significantly associated with a 2.49-fold increased risk of cervical cancer. The significant increase in IL-1β concentration in cervical cancer cases was observed only among cervical cancer cases carrying C-511T variant genotypes. T/T genotype of IL-1β polymorphism was significantly higher in cervical cancer cases compared with controls (57 vs. 38%; OR = 2.16; P = 0.028) and the T allele carriage was significantly associated with cervical cancer risk (OR = 2.00, 95% CI = 1.19-3.38, and P = 0.008). In conclusion, plasma IL-1β level and IL-1β C-511T polymorphism may be considered as candidate biomarkers for cervical cancer in Egyptian women. |
| Databáze: | OpenAIRE |
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