Site-Specific Direct Labeling of Neurotrophins and Their Receptors: From Biochemistry to Advanced Imaging Applications
| Autor: | Fulvio Bonsignore, Laura Marchetti, Rosy Amodeo, Antonino Cattaneo, Francesco Gobbo |
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| Přispěvatelé: | Gobbo F., Bonsignore F., Amodeo R., Cattaneo A., Marchetti L., Skaper S., Gobbo, Francesco, Bonsignore, Fulvio, Amodeo, Rosy, Cattaneo, Antonino, Marchetti, Laura |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Inducible lentiviru Context (language use) Fluorescence labeling Living cell Computational biology Astrocyte culture 03 medical and health sciences Nerve growth factor Neurotrophin Nerve growth factor TrkA tyrosine kinase Membrane receptor pool Fluorescence labeling Biotinylation Axonal transport Inducible lentivirus Neuron culture Astrocyte culture Genetics Inducible lentivirus Biotinylation Receptor Molecular Biology Axonal transport biology Chemistry Membrane receptor pool TrkA tyrosine kinase 030104 developmental biology Neuron culture biology.protein Neurotrophin |
| Zdroj: | Neurotrophic Factors ISBN: 9781493975709 |
| DOI: | 10.1007/978-1-4939-7571-6_22 |
| Popis: | We describe here a versatile methodological platform to achieve site-directed and stoichiometry-controlled labeling of neurotrophins and their receptors with various probes, ranging from biotin to small organic dyes. This labeling method works in vitro on purified neurotrophins as well as in a living cell context, where it achieves selective labeling of surface-exposed neurotrophin receptors. Here, we list all experimental details of our labeling protocols, along with examples of the wide range of applications in which these can be used. |
| Databáze: | OpenAIRE |
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