Regulation of ptsH and ptsl gene expression in Streptococcus salivarius ATCC 25975
| Autor: | Christian Vadeboncoeur, Michel Frenette, Lucie Gauthier, Guy Gagnon |
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| Rok vydání: | 1995 |
| Předmět: |
Transcription
Genetic Operon Blotting Western Molecular Sequence Data Mutant Biology Microbiology Bacterial Proteins Transcription (biology) Gene expression Transcriptional regulation Amino Acid Sequence RNA Messenger Phosphoenolpyruvate Sugar Phosphotransferase System Promoter Regions Genetic Molecular Biology Gene DNA Primers Terminator Regions Genetic Genetics Base Sequence Single-Strand Specific DNA and RNA Endonucleases Phosphotransferases (Nitrogenous Group Acceptor) Streptococcus Gene Expression Regulation Bacterial PEP group translocation Blotting Northern biology.organism_classification Molecular biology Streptococcus salivarius Mutation |
| Zdroj: | Molecular Microbiology. 16:1111-1121 |
| ISSN: | 1365-2958 0950-382X |
| DOI: | 10.1111/j.1365-2958.1995.tb02336.x |
| Popis: | Summary The transcriptional regulation of the Streptococcus salivarius ptsH and ptsl genes coding for the general energy-coupling proteins HPr and enzyme I of the phosphoenolpyruvate:sugar phosphotransferase system were investigated. These genes form an operon with the gene order ptsH–ptsl. Three distinct mRNA species were detected: a 0.5 kb transcript specific for ptsH, and two long transcripts (2.2 arid 2.4 kb) covering the whole pts operon. Transcription of all these mRNAs initiated at the same nucleotide located 9 bp downstream from a promoter located immediately upstream from the ptsH gene. The presence of a high-energy stem–loop structure (T0) located at the begining of ptsl was responsible for the premature transcrrption termination generating the 0.5 kb ptsH-specific transcript. The long transcripts ended in the poly(U) region of two rho-independent-like terminators (T1 and T2) at the 3′ end of ptsl. Studies with a 2-deoxyglucose-resistant spontaneous mutant of S. salivarius (L26) that produces an HPr–El fusion protein suggest that the regulation of HPr and El expression involves transcriptional as well as translational mechanisms. |
| Databáze: | OpenAIRE |
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