Human hematopoietic survival and growth factor
| Autor: | Tadashi Ohkubo, Atsunobu Hiraoka, Minoru Fukuda |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
medicine.medical_specialty
Cell Survival medicine.medical_treatment Granulocyte Biology Colony-Stimulating Factors Internal medicine medicine Leukocytes Humans Cells Cultured Growth factor Macrophages Cell Biology Colony-stimulating factor Chromatography Ion Exchange Hematopoietic Stem Cells In vitro Culture Media Haematopoiesis Endocrinology medicine.anatomical_structure Cell culture Tetradecanoylphorbol Acetate Cancer research Chromatography Gel Stem cell |
| Zdroj: | Cell biology international reports. 10(5) |
| ISSN: | 0309-1651 |
| Popis: | A variety of biologic activities on human hematopoietic stem cells were detected in 12-O-tetradecanoylphorbol-13-acetate-stimulated leukocyte-conditioned medium (TPA-LCM). Partial purification of TPA-LCM demonstrated the presence of human-active granulocyte-macrophage colony-stimulating factor (GM-CSF; Mr 50-70Kd), macrophage-CSF (M-CSF; Mr greater than 100Kd) and possibly a third protein (Mr 24-26Kd) supporting survival and growth of hematopoietic stem cells in vitro. Activities investigated included GM-colony and erythroid burst-potentiation, burst-promoting activity (BPA) and delta granulocyte and delta burst-promoting activities (delta GPA and delta BPA). |
| Databáze: | OpenAIRE |
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