Translational Landscape of Photomorphogenic Arabidopsis
| Autor: | Jing-Fen Wu, Huang-Lung Tsai, Wen-Dar Lin, Szu-Hsien Wu, Ming-Jung Liu, Yi-Chen Wu, Shu-Hsing Wu, Tsung-Ying Tsai |
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| Rok vydání: | 2013 |
| Předmět: |
Light
Arabidopsis Codon Initiator Plant Science Ribosome Open Reading Frames Gene Expression Regulation Plant Translational regulation Morphogenesis Protein biosynthesis Arabidopsis thaliana RNA Messenger Large-Scale Biology Article Ribosome profiling Gene Library Genetics biology Sequence Analysis RNA Chromosome Mapping Translation (biology) Cell Biology biology.organism_classification MicroRNAs Open reading frame RNA Plant Protein Biosynthesis Ribosomes |
| Zdroj: | The Plant Cell. 25:3699-3710 |
| ISSN: | 1532-298X 1040-4651 |
| Popis: | Translational control plays a vital role in regulating gene expression. To decipher the molecular basis of translational regulation in photomorphogenic Arabidopsis thaliana, we adopted a ribosome profiling method to map the genome-wide positions of translating ribosomes in Arabidopsis etiolated seedlings in the dark and after light exposure. We found that, in Arabidopsis, a translating ribosome protects an ~30-nucleotide region and moves in three-nucleotide periodicity, characteristics also observed in Saccharomyces cerevisiae and mammals. Light enhanced the translation of genes involved in the organization and function of chloroplasts. Upstream open reading frames initiated by ATG but not CTG mediated translational repression of the downstream main open reading frame. Also, we observed widespread translational repression of microRNA target genes in both light- and dark-grown Arabidopsis seedlings. This genome-wide characterization of transcripts undergoing translation at the nucleotide-resolution level reveals that a combination of multiple translational mechanisms orchestrates and fine-tunes the translation of diverse transcripts in plants with environmental responsiveness. |
| Databáze: | OpenAIRE |
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