Methionine sulfoxide reductase A (MsrA) contributes to Salmonella Typhimurium survival against oxidative attack of neutrophils
| Autor: | Manoj Kumawat, Tapas Kumar Goswami, Manish Mahawar, Pavan Kumar Pesingi, Raj Narayan Trivedi, Vivek Kumar Gupta, Pranjali Agarwal |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Salmonella typhimurium
Salmonella Neutrophils Immunology Virulence Oxidative phosphorylation Biology medicine.disease_cause Microbiology chemistry.chemical_compound Gene Order medicine Immunology and Allergy Peroxidase Sequence Deletion Methionine Microbial Viability Methionine sulfoxide Genetic Complementation Test Hematology Hydrogen Peroxide Oxidative Stress chemistry Genetic Loci Myeloperoxidase Methionine Sulfoxide Reductases biology.protein Oxidative stress MSRA |
| Zdroj: | Immunobiology. 220(12) |
| ISSN: | 1878-3279 |
| Popis: | Salmonella Typhimurium (ST) must evade neutrophil assault for infection establishment in the host. Myeloperoxidase generated HOCl is the key antimicrobial agent produced by the neutrophils; and methionine (Met) residues are the primary targets of this oxidant. Oxidation of Mets leads to methionine sulfoxide (Met-SO) formation and consequently compromises the protein function(s). Methionine sulfoxide reductase A (MsrA) reductively repairs Met-SO to Mets. In this manner, MsrA maintains the function(s) of key proteins which are important for virulence of ST and enhance the survival of this bacterium under oxidative stress. We constructed msrA gene deletion strain (ΔmsrA). The primers located in the flanking regions to ΔmsrA gene amplified 850 and 300 bp amplicons in ST and ΔmsrA strains, respectively. The ΔmsrA strain grew normally in in vitro broth culture. However, ΔmsrA strain showed high susceptibility (p0.001) to very low concentrations of HOCl which was restored (at least in part) by plasmid based complementation. ΔmsrA strain was hypersensitive (than ST) to the granules isolated from neutrophils. Further, the ΔmsrA strain was significantly (p0.05) more susceptible to neutrophil mediated killing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect