Myxococcus xanthus Encapsulin as a Promising Platform for Intracellular Protein Delivery

Autor:	Anna N. Gabashvili, Nelly S. Chmelyuk, Viktoria A. Sarkisova, Pavel A. Melnikov, Alevtina S. Semkina, Aleksey A. Nikitin, Maxim A. Abakumov
Rok vydání:	2022
Předmět:	Inorganic Chemistry Organic Chemistry encapsulins fluorescence photoactivatable label protein delivery General Medicine Physical and Theoretical Chemistry Molecular Biology Spectroscopy Catalysis Computer Science Applications
Zdroj:	International Journal of Molecular Sciences; Volume 23; Issue 24; Pages: 15591
ISSN:	1422-0067
DOI:	10.3390/ijms232415591
Popis:	Introducing a new genetically encoded material containing a photoactivatable label as a model cargo protein, based on Myxococcus xanthus (Mx) encapsulin system stably expressed in human 293T cells. Encapsulin from Mx is known to be a protein-based container for a ferritin-like cargo in its shell which could be replaced with an exogenous cargo protein, resulting in a modified encapsulin system. We replaced Mx natural cargo with a foreign photoactivatable mCherry (PAmCherry) fluorescent protein and isolated encapsulins, containing PAmCherry, from 293T cells. Isolated Mx encapsulin shells containing photoactivatable label can be internalized by macrophages, wherein the PAmCherry fluorescent signal remains clearly visible. We believe that a genetically encoded nanocarrier system obtained in this study, can be used as a platform for controllable delivery of protein/peptide therapeutics in vitro.
Databáze:	OpenAIRE
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