Synthesis and analysis of activity of a potential anti-melanoma prodrug with a hydrazine linker
| Autor: | Beata Gąsowska-Bajger, Bożena Frąckowiak-Wojtasek, Stanisław Szala, Marieke Logghe, Hubert Wojtasek, Ryszard Smolarczyk, Magdalena Mazurek, Agnieszka Raniszewska, Tomasz Cichoń |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Melphalan
Stereochemistry Tyrosinase Hydrazine Melanoma Experimental tyrosinase chemistry.chemical_compound Mice Nucleophile Cell Line Tumor Drug Discovery medicine Animals Prodrugs Mechlorethamine Tyrosine neoplasms Pharmacology anti-melanoma prodrug Chemistry Monophenol Monooxygenase Organic Chemistry nitrogen mustard hydrazine General Medicine Prodrug Nitrogen mustard Hydrazines Cyclization Linker medicine.drug |
| Zdroj: | European Journal of Medicinal Chemistry. 71:98-104 |
| ISSN: | 0223-5234 |
| Popis: | A potential anti-melanoma prodrug containing a phenolic activator, a hydrazine linker, and a nitrogen mustard effector - (N-{4-[bis-(2-chloroethyl)amino]benzoyl}-N'-(4-hydroxybenzyl)hydrazine) has been synthesized in seven steps. Spectrophotometric measurements of its oxidation by tyrosinase showed a rapid increase of absorbance at 337 nm. HPLC analysis demonstrated that two major products were formed. However, during the reaction one of the products was converted into the other. The stable product with a maximum of absorption at 337 nm was isolated and identified as 5,6-dihydroxy-1H-indazol-1-yl 4-[bis-(2-chloroethyl)amino]benzoate. It was formed by a cyclization of the enzymatically generated o-quinone. This reaction was unexpected, since the acylated hydrazine nitrogen atom should not be sufficiently nucleophilic to attack the o-quinone ring. This cyclization prevented the effector release from the enzyme-activated prodrug. As a result, the prodrug showed only limited specificity for B16-F10 murine melanoma cells compared to reference cell lines. When applied in solid tumors in mice it showed slightly higher activity than the parent mustard drug (4-[bis-(2-chloroethyl)amino]benzoic cid), but significantly lower activity than melphalan, a commercial mustard drug with a structure resembling tyrosine, occasionally used in the treatment of melanoma. |
| Databáze: | OpenAIRE |
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