Desulfobulbus oligotrophicus sp. nov., a sulfate-reducing and propionate-oxidizing bacterium isolated from a municipal anaerobic sewage sludge digester
| Autor: | Adrien Dakdaki, Radia Bouterfass, Abdelaziz El Houari, Abdel-Illah Qatibi, Lahcen Idouhammou, Anthony Ranchou-Peyruse, Rémy Guyoneaud, Magali Ranchou-Peyruse, Rhizlane Bennisse, Marion Guignard |
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| Přispěvatelé: | Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Génie Microbiologique [Marrakech], Université Cadi Ayyad [Marrakech] (UCA) |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Deltaproteobacteria 010501 environmental sciences Biology 01 natural sciences Microbiology 03 medical and health sciences chemistry.chemical_compound sulfate reduction RNA Ribosomal 16S [CHIM]Chemical Sciences disproportionation of sulfite Ecology Evolution Behavior and Systematics Phylogeny 0105 earth and related environmental sciences chemistry.chemical_classification Thiosulfate Base Composition Sewage Sulfur-Reducing Bacteria Fatty Acids Desulfobulbus General Medicine Sequence Analysis DNA biology.organism_classification 16S ribosomal RNA 6. Clean water Bacterial Typing Techniques Morocco sludge digester 030104 developmental biology Desulfobulbus propionicus chemistry Propionate Fermentation Propionates Oxidation-Reduction Bacteria Sludge Mesophile |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2017, 67 (2), pp.275--281. ⟨10.1099/ijsem.0.001615⟩ |
| ISSN: | 1466-5034 1466-5026 |
| DOI: | 10.1099/ijsem.0.001615⟩ |
| Popis: | International audience; A novel, mesophilic, strictly anaerobic, sulfate-reducing and propionate-oxidizing bacterium, strain Prop6T, was enriched and isolated from a municipal anaerobic sewage sludge digester. Cells were Gram-stain-negative, catalase-positive, oval rods, motile by means of amphitrichous flagella, non-spore-forming and contained menaquinone MK-5(H2) as the major respiratory quinone. The genomic DNA G+C content was 51.7 mol%. The optimal NaCl concentration, temperature and pH were 2–5 g l−1, 35 °C and pH 7.6, respectively. Strain Prop6T could only oxidize propionate, lactate and pyruvate (weakly) with sulfate, sulfite or thiosulfate, mainly to acetate. Strain Prop6T fermented pyruvate and lactate to acetate and propionate. The predominant cellular fatty acids were C14 : 0, C16 : 0, C16 : 1ω7, C16 : 1ω5, C17 : 1ω6 and C18 : 1ω7. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the newly isolated strain was a member of the genus Desulfobulbus , with Desulfobulbus elongatus DSM 2908T, Desulfobulbus propionicus DSM 2032T and Desulfobulbus rhabdoformis DSM 8777T as closest relatives among species with validly published names. On the basis of genotypic, phenotypic and chemotaxonomic characteristics, it is proposed that the isolate represents a novel species, Desulfobulbus oligotrophicus sp. nov. The type strain is Prop6T (=DSM 103420T=JCM 31535T). |
| Databáze: | OpenAIRE |
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