Application of an efficient strategy for discovery and purification of bioactive compounds from Chinese herbal medicines, a case study on the Puerariae thomsonii Flos
| Autor: | Chi-Yu Ge, Xiao-Lan Cheng, Qi Wang, Minjian Qin, Huan Li, Xiao-Ying Qin, Rui Liu, Lian-Wen Qi |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Tectorigenin Spectrometry Mass Electrospray Ionization Electrospray ionization Clinical Biochemistry Pharmaceutical Science Flos Tectoridin Mass spectrometry Tandem mass spectrometry Analytical Chemistry Inhibitory Concentration 50 Mice chemistry.chemical_compound Tandem Mass Spectrometry Cell Line Tumor Drug Discovery Animals Glycosides Countercurrent Distribution Chromatography High Pressure Liquid Spectroscopy chemistry.chemical_classification Chromatography Molecular Structure biology Prostatic Neoplasms Glycoside biology.organism_classification Antineoplastic Agents Phytogenic Isoflavones Pueraria chemistry Ethnopharmacology Drug Screening Assays Antitumor Lead compound Drugs Chinese Herbal |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 75:25-32 |
| ISSN: | 0731-7085 |
| DOI: | 10.1016/j.jpba.2012.11.009 |
| Popis: | In this study, an efficient strategy based on bioassay-guided fractionation, high-performance liquid chromatography/electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC-ESI-Q/TOF-MS) and high-speed counter-current chromatography (HSCCC) was established to screen and purify bioactive compounds from Chinese herbal medicines (CHMs). This screening system was efficient and successfully applied to reveal anti-prostate cancer candidates from Puerariae thomsonii Flos. As a result, an active fraction with strong in vitro anti-prostate cancer activity was obtained, and the main compounds in the fraction were purified by HSCCC, giving 82 mg of tectoridin, 36 mg of tectorigenin-7-O-[β-D-xylopyranosyl-(1→6)-β-D-glucopyranoside and 64 mg of tectorigenin. Among them, tectorigenin, possessing the highest anti-prostate cancer activity with IC₅₀ value of 0.08 μM, has priority to be lead compound. The results of this work demonstrated that the developed method was efficient and could be employed for the rapid screening, identification and purification of active components from CHMs. |
| Databáze: | OpenAIRE |
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