Molecular Imaging Reveals a Progressive Pulmonary Inflammation in Lower Airways in Ferrets Infected with 2009 H1N1 Pandemic Influenza Virus

Autor: Yong-Kyu Chu, Leslie C. Sherwood, Chin K. Ng, Ashley E. Biller, Jennifer L. Kraenzle, Huaiyu Zheng, Marlene C. Steffen, Albert Wu, Carol D. Vanover, Mary Proctor, Jeremy V. Camp, Daniel J. Mollura, Colleen B. Jonsson
Rok vydání: 2012
Předmět:
RNA viruses
Pathology
Viral Diseases
PET imaging
lcsh:Medicine
Virus Replication
Multimodal Imaging
Diagnostic Radiology
Influenza A Virus
H1N1 Subtype
Viral classification
lcsh:Science
Lung
Nose
Multidisciplinary
medicine.diagnostic_test
Molecular Imaging
Virus Shedding
medicine.anatomical_structure
Infectious Diseases
Positron emission tomography
Disease Progression
Medicine
Female
medicine.symptom
Radiology
Research Article
medicine.medical_specialty
Immunology
Inflammation
Microbiology
Virus
Computed Tomography
Orthomyxoviridae Infections
Fluorodeoxyglucose F18
Throat
Virology
medicine
Animals
Viral shedding
Pandemics
Biology
business.industry
lcsh:R
Ferrets
Immunity
Radiobiology
Pneumonia
Influenza
Animal Models of Infection
Viral replication
Positron-Emission Tomography
Nuclear medicine
lcsh:Q
business
Tomography
X-Ray Computed
Zdroj: PLoS ONE
PLoS ONE, Vol 7, Iss 7, p e40094 (2012)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0040094
Popis: Molecular imaging has gained attention as a possible approach for the study of the progression of inflammation and disease dynamics. Herein we used [(18)F]-2-deoxy-2-fluoro-D-glucose ([(18)F]-FDG) as a radiotracer for PET imaging coupled with CT (FDG-PET/CT) to gain insight into the spatiotemporal progression of the inflammatory response of ferrets infected with a clinical isolate of a pandemic influenza virus, H1N1 (H1N1pdm). The thoracic regions of mock- and H1N1pdm-infected ferrets were imaged prior to infection and at 1, 2, 3 and 6 days post-infection (DPI). On 1 DPI, FDG-PET/CT imaging revealed areas of consolidation in the right caudal lobe which corresponded with elevated [(18)F]-FDG uptake (maximum standardized uptake values (SUVMax), 4.7-7.0). By days 2 and 3, consolidation (CT) and inflammation ([(18)F]-FDG) appeared in the left caudal lobe. By 6 DPI, CT images showed extensive areas of patchy ground-glass opacities (GGO) and consolidations with the largest lesions having high SUVMax (6.0-7.6). Viral shedding and replication were detected in most nasal, throat and rectal swabs and nasal turbinates and lungs on 1, 2 and 3 DPI, but not on day 7, respectively. In conclusion, molecular imaging of infected ferrets revealed a progressive consolidation on CT with corresponding [(18)F]-FDG uptake. Strong positive correlations were measured between SUVMax and bronchiolitis-related pathologic scoring (Spearman's ρ = 0.75). Importantly, the extensive areas of patchy GGO and consolidation seen on CT in the ferret model at 6 DPI are similar to that reported for human H1N1pdm infections. In summary, these first molecular imaging studies of lower respiratory infection with H1N1pdm show that FDG-PET can give insight into the spatiotemporal progression of the inflammation in real-time.
Databáze: OpenAIRE
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