T. brucei RNA editing: adenosine nucleotides inversely affect U-deletion and U-insertion reactions at mRNA cleavage
| Autor: | Jorge Cruz-Reyes, Barbara Sollner-Webb, K.J. Piller, Laura N. Rusché |
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| Rok vydání: | 1998 |
| Předmět: |
Cleavage factor
Adenosine Allosteric regulation Trypanosoma brucei brucei Biology Cleavage (embryo) Adenosine Triphosphate medicine RNA Precursors Animals Nucleotide Molecular Biology chemistry.chemical_classification Base Sequence MRNA cleavage RNA Ligase (ATP) RNA Nucleotidyltransferases Cell Biology Cytochrome b Group Endonucleases Adenosine Diphosphate Biochemistry chemistry RNA editing Duplex (building) Mutagenesis Site-Directed RNA Editing RNA Protozoan medicine.drug |
| Zdroj: | Molecular cell. 1(3) |
| ISSN: | 1097-2765 |
| Popis: | In the currently envisioned mechanism of trypanosome mitochondrial RNA editing, U-insertion and U-deletion cycles begin with a common kind of gRNA-directed cleavage. However, natural, altered, and mutationally interconverted editing sites reveal that U-deletional cleavage is inefficient without and activated by ATP and ADP, while U-insertional cleavage shows completely reverse nucleotide effects. The adenosine nucleotides' effects appear to be allosteric and determined solely by sequences immediately adjacent to the anchor duplex. Both U-deletional and U-insertional cleavages are reasonably active at physiological mitochondrial ATP concentration. Notably, ATP and ADP markedly stimulate complete U-deletion and inhibit U-insertion reactions, reflecting their effects on cleavage. These plus previous results suggest that U deletion and U insertion are remarkably distinct. |
| Databáze: | OpenAIRE |
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