Positive regulation by protein kinase C of slow Na current in Xenopus oocytes
| Autor: | Nathalie Behue, Franck Fournier, Gilles Charpentier, D. Marlot, Gérard Brûlé |
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| Rok vydání: | 1993 |
| Předmět: |
Xenopus
Tetrodotoxin General Biochemistry Genetics and Molecular Biology Sodium Channels Membrane Potentials Diglycerides Xenopus laevis Alkaloids Phorbol Esters medicine Animals Protein phosphorylation Protein kinase A Protein kinase C Protein Kinase C General Environmental Science General Immunology and Microbiology biology Depolarization General Medicine biology.organism_classification Oocyte Staurosporine Cell biology Enzyme Activation Tamoxifen medicine.anatomical_structure Biochemistry Oocytes Phosphorylation Alkaline phosphatase Tetradecanoylphorbol Acetate Female General Agricultural and Biological Sciences |
| Zdroj: | Proceedings. Biological sciences. 254(1339) |
| ISSN: | 0962-8452 |
| Popis: | The slow inward Na current observed during sustained depolarization of the Xenopus oocyte membrane is due to a complex mechanism described as the induction of the channels. The present work investigates the role of protein phosphorylation in Na channel function. Injection of alkaline phosphatase in the oocytes decreased inward current. Therefore, the possible involvement of protein kinase in Na channel induction was explored. Treatment of oocytes with two activators of protein kinase C (PKC) resulted in enhanced Na current amplitude, whereas the treatment of oocytes with two potent PKC inhibitors decreased the inward current. These results imply that PKC phosphorylation is a fundamental step of Na channel induction. The possibility that the depolarization of the oocyte membrane may be the factor involved in PKC activation is discussed. |
| Databáze: | OpenAIRE |
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