Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture
| Autor: | John T.Y. Wu, Xiaomei Li, Evelyn E. Bowlby, Lester S. Y. Wong, Xiaoli Pang, Yupin Tong, Tiejun Gao, Robert J. Tower |
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| Rok vydání: | 2012 |
| Předmět: |
Infectivity
Environmental Engineering Renewable Energy Sustainability and the Environment Thermophile Bioengineering General Medicine Biology Real-Time Polymerase Chain Reaction Virology Virus Microbiology Matrix (chemical analysis) Real-time polymerase chain reaction Cytopathogenic Effect Viral Herpesvirus 1 Gallid Cell culture Cell Line Tumor Nucleic acid Humans Anaerobiosis Fragmentation (cell biology) Waste Management and Disposal |
| Zdroj: | Bioresource Technology. 110:692-696 |
| ISSN: | 0960-8524 |
| Popis: | The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5 h in experiment 1 and for 16 h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p < 0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5 h-treatment and was not present by 16 h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses. |
| Databáze: | OpenAIRE |
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