| Autor: |
Jake McClements, Laure Bar, Pankaj Singla, Francesco Canfarotta, Alan Thomson, Joanna Czulak, Rhiannon E. Johnson, Robert D. Crapnell, Craig E. Banks, Brendan Payne, Shayan Seyedin, Patricia Losada-Pérez, Marloes Peeters |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
ACS sensors. 7(4) |
| ISSN: |
2379-3694 |
| Popis: |
Rapid antigen tests are currently used for population screening of COVID-19. However, they lack sensitivity and utilize antibodies as receptors, which can only function in narrow temperature and pH ranges. Consequently, molecularly imprinted polymer nanoparticles (nanoMIPs) are synthetized with a fast (2 h) and scalable process using merely a tiny SARS-CoV-2 fragment (∼10 amino acids). The nanoMIPs rival the affinity of SARS-CoV-2 antibodies under standard testing conditions and surpass them at elevated temperatures or in acidic media. Therefore, nanoMIP sensors possess clear advantages over antibody-based assays as they can function in various challenging media. A thermal assay is developed with nanoMIPs electrografted onto screen-printed electrodes to accurately quantify SARS-CoV-2 antigens. Heat transfer-based measurements demonstrate superior detection limits compared to commercial rapid antigen tests and most antigen tests from the literature for both the alpha (∼9.9 fg mL |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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