Real-time observation of protein aggregates in pharmaceutical formulations using liquid cell electron microscopy
| Autor: | Kaya Patel, Jonathan Haulenbeek, Yanping Liang, Madeline J. Dukes, Songyan Zheng, Mario Hubert, Steven P. Piccoli, Lynn M. DiMemmo, Deborah F. Kelly, A. Cameron Varano |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Time Factors
Solution state Protein Conformation Drug Compounding Biomedical Engineering Bioengineering Nanotechnology 02 engineering and technology Protein aggregation 010402 general chemistry 01 natural sciences Biochemistry Article law.invention Polyethylene Glycols Protein Aggregates law Native state Chemistry Direct observation A protein Interferon-alpha General Chemistry 021001 nanoscience & nanotechnology 0104 chemical sciences Microscopy Electron Liquid cell Electron microscope 0210 nano-technology Function (biology) |
| Zdroj: | Lab on a chip. 17(2) |
| ISSN: | 1473-0189 |
| Popis: | Understanding the properties of protein-based therapeutics is a common goal of biologists and physicians. Technical barriers in the direct observation of small proteins or therapeutic agents can limit our knowledge of how they function in solution and in the body. Electron microscopy (EM) imaging performed in a liquid environment permits us to peer into the active world of cells and molecules at the nanoscale. Here, we employ liquid cell EM to directly visualize a protein-based therapeutic in its native conformation and aggregate state in a time-resolved manner. In combination with quantitative analyses, information from this work contributes new molecular insights toward understanding the behaviours of immunotherapies in a solution state that mimics the human body. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|