Characterization of two new cuticular genes specifically expressed during the post-ecdysial molting period in Tenebrio molitor
| Autor: | Jocelyne Mathelin, Brigitte Quennedey, Hervé Bouhin, Jean Delachambre |
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| Rok vydání: | 1998 |
| Předmět: |
Signal peptide
Molecular Sequence Data Gene Expression Genes Insect Molting Biology Complementary DNA Genetics Animals Genomic library Amino Acid Sequence RNA Messenger Tenebrio Gene Histidine Messenger RNA Genome Base Sequence Sequence Homology Amino Acid Pupa Intron Gene Expression Regulation Developmental DNA Sequence Analysis DNA General Medicine Molecular biology Genes Biochemistry Larva Insect Proteins Moulting |
| Zdroj: | Gene. 211:351-359 |
| ISSN: | 0378-1119 |
| DOI: | 10.1016/s0378-1119(98)00125-5 |
| Popis: | In a previous study, we have isolated a cDNA, TM-ACP17 , coding for a post-ecdysial adult protein of Tenebrio molitor . After screening of a genomic library with TM-ACP17 , we report isolation and sequencing of TM-ACP17 gene and a new gene, TM-LPCP29 , coding for a larval–pupal protein. These two genes exhibit a common sequence of 15 nucleotides and a characteristic of most cuticular protein genes so far described: an intron interrupting the signal peptide. The deduced aa sequence of TM-LPCP29 exhibits a high percentage of Ala (26.5%) and Val (17.5%) and is highly hydrophobic. In the N-terminal part, the motif VAAPV is repeated ten times. Numerous histidine residues are present in the C- and N-terminal regions. A comparison is made with other cuticle protein sequences. Northern hybridization analysis showed that TM-LPCP29 is present during larval and mainly pupal post-ecdysial cuticle secretion. In-situ hybridization revealed that TM-LPCP29 mRNA is expressed in epidermis and not in muscles or fat body. |
| Databáze: | OpenAIRE |
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