Honeycomb form β-tricalcium phosphate induces osteogenesis by geometrical property with BMSC
| Autor: | Rachel Sammons, Kimitoshi Yagami, Satoru Shintani, Tatsuo Shirota, Yoshinori Kuboki, Mitsuori Mayahara, Mikio Nishizawa, Shigeru Yanagisawa |
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| Rok vydání: | 2011 |
| Předmět: |
Calcium Phosphates
Scaffold Artificial bone Materials science Surface Properties Molecular Conformation Biomedical Engineering Bone Marrow Cells Models Biological Bone augmentation Biomaterials chemistry.chemical_compound Dogs Osteogenesis medicine Animals Composite material Cells Cultured β tricalcium phosphate Mesenchymal stem cell Mesenchymal Stem Cells General Medicine medicine.disease Phosphate In vitro Up-Regulation Cell biology chemistry Female Porosity Calcification |
| Zdroj: | Bio-Medical Materials and Engineering. 21:291-306 |
| ISSN: | 0959-2989 |
| DOI: | 10.3233/bme-2012-0677 |
| Popis: | To establish an effective method for bone augmentation, we introduced a new honeycomb-like β-tricalcium phosphate (H-β-TCP) with BMP-2 as a scaffold, whose unique geometrical properties induce osteoblastic differentiation of autologous bone marrow mesenchymal stem cells (BMSCs). A total of six beagle dogs from 6 to 7 years old were used for this study. BMSCs were cultured with autologous serum and BMP-2 on H-β-TCP. Differentiation to osteoblasts was demonstrated in vitro and exo vivo. Scanning electron microscopy revealed formation and calcification of a matrix-like structure within the H-β-TCP tunnels in BMSC culture. Moreover, treatment of BMP-2 promoted osteoblastic differentiation of BMSCs in H-β-TCP in a diffusion chamber. These results indicated that H-β-TCP may be a useful tool for construction of functional artificial bone. |
| Databáze: | OpenAIRE |
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