S. cerevisiae chromosomes biorient via gradual resolution of syntely between S phase and anaphase
| Autor: | Jonas F. Dorn, Gaudenz Danuser, Khuloud Jaqaman, Peter K. Sorger, Eugenio Marco, Pei Hsin Hsu |
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| Rok vydání: | 2012 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Biorientation Saccharomyces cerevisiae Spindle Apparatus Biology Protein Serine-Threonine Kinases General Biochemistry Genetics and Molecular Biology S Phase 03 medical and health sciences 0302 clinical medicine Aurora Kinases Sister chromatids Humans Prometaphase Kinetochores 030304 developmental biology Anaphase 0303 health sciences Cohesin Biochemistry Genetics and Molecular Biology(all) Intracellular Signaling Peptides and Proteins Cell biology Spindle apparatus Establishment of sister chromatid cohesion Spindle checkpoint Chromosomes Fungal 030217 neurology & neurosurgery |
| Zdroj: | Cell. 154(5) |
| ISSN: | 1097-4172 |
| Popis: | SummaryFollowing DNA replication, eukaryotic cells must biorient all sister chromatids prior to cohesion cleavage at anaphase. In animal cells, sister chromatids gradually biorient during prometaphase, but current models of mitosis in S. cerevisiae assume that biorientation is established shortly after S phase. This assumption is based on the observation of a bilobed distribution of yeast kinetochores early in mitosis and suggests fundamental differences between yeast mitosis and mitosis in animal cells. By applying super-resolution imaging methods, we show that yeast and animal cells share the key property of gradual and stochastic chromosome biorientation. The characteristic bilobed distribution of yeast kinetochores, hitherto considered synonymous for biorientation, arises from kinetochores in mixed attachment states to microtubules, the length of which discriminates bioriented from syntelic attachments. Our results offer a revised view of mitotic progression in S. cerevisiae that augments the relevance of mechanistic information obtained in this powerful genetic system for mammalian mitosis. |
| Databáze: | OpenAIRE |
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