Lack of inducible nitric oxide synthases attenuates leukocyte-endothelial cell interactions in retinal microcirculation
| Autor: | Daisuke Iwama, Fumitaka Hirose, Nagahisa Yoshimura, Kazuaki Miyamoto, S. Miyahara, Hiroshi Tamura |
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| Rok vydání: | 2008 |
| Předmět: |
Lipopolysaccharides
Male Lipopolysaccharide Nitric Oxide Synthase Type II Leukocyte Rolling Cell Communication Biology Nitric oxide Microcirculation Uveitis Cellular and Molecular Neuroscience chemistry.chemical_compound Leukocyte Count Mice Cell–cell interaction Cell Movement Image Processing Computer-Assisted Leukocytes Animals Fluorometry Fluorescent Dyes Mice Knockout Acridine orange Endothelial Cells Retinal Vessels Original Articles Molecular biology Sensory Systems eye diseases Acridine Orange Laser Scanning Cytometry Endothelial stem cell Nitric oxide synthase Mice Inbred C57BL Ophthalmology Laboratory Science chemistry Immunology Models Animal biology.protein sense organs |
| Zdroj: | The British Journal of Ophthalmology |
| ISSN: | 1468-2079 |
| Popis: | Aim: To investigate the effect of inducible nitric oxide synthases (iNOS) on inflammatory reactions during endotoxin-induced uveitis (EIU) in mice by studying leukocyte–endothelial cell interactions. Methods: EIU was produced in immunosuppressed iNOS−/− mice and C57BL/6 (normal) mice by footpad injection of lipopolysaccharide. Leukocytes were labelled with acridine orange. Leukocyte rolling in the retinal microcirculation was evaluated in vivo with acridine orange digital fluorography. The number of migrated leukocytes was counted in flat-mounted retina. Results: Both leukocyte rolling and migration peaked at 48 h after lipopolysaccharide injection. The maximal numbers of rolling leukocytes in the immunosuppressed iNOS−/− mouse retina decreased by 98.2% (p |
| Databáze: | OpenAIRE |
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