Lrp as a potential transcriptional regulator involved in stress response in Haloferax mediterranei

Autor: Laura Matarredona, Mónica Camacho, Vanesa Bautista, María-José Bonete, Julia Esclapez
Přispěvatelé: Universidad de Alicante. Departamento de Bioquímica y Biología Molecular y Edafología y Química Agrícola, Biotecnología de Extremófilos (BIOTECEXTREM)
Rok vydání: 2023
Předmět:
Zdroj: Biochimie. 209:61-72
ISSN: 0300-9084
DOI: 10.1016/j.biochi.2023.01.012
Popis: The Archaea domain consists of a heterogeneous group of microorganisms with unique physiological properties that occupy a wide variety of niches in nature. Haloferax mediterranei is an extremely halophilic archaeon classified in the Phylum Euryarchaeota, which requires a high concentration of inorganic salts for optimal growth. In haloarchaea, transcription factors play a fundamental role in an adequate adaptation to environmental and nutritional changes, preserving the survival and integrity of the organism. To deepen knowledge of the Lrp/AsnC transcriptional regulator family, a lrp gene (HFX_RS01210) from this family has been studied. Site-directed mutagenesis has allowed us to identify the TATA-box and two potential sites of the transcriptional factor (TF) to its own promoter and autoregulate itself. Several approaches were carried out to elucidate whether this transcriptional regulator is involved in stresses due to heavy metals and limited nitrogen conditions. Characterization of the lrp deletion mutant and the Lrp overexpressed strain, suggests that the level of lrp expression depends on the nitrogen source and the presence of cobalt. The most striking results were obtained in the presence of nitrate as a nitrogen source due to the inability of the deletion mutant to grow. All these results confirm that Lrp is a powerful candidate for a regulatory role in the stress response, particularly under N-limiting conditions and the presence of cobalt. This work was supported by Programa Propio para el Fomento de la I + D + I del Vicerrectorado de Investigación y Transferencia de Conocimiento” of the University of Alicante (VIGRO-016).
Databáze: OpenAIRE
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