The extended AT-hook is a novel RNA binding motif
| Autor: | Karina Zillner, Michael Filarsky, Ana Villar-Garea, Gernot Längst, Rainer Merkl, Attila Németh, Ingrid Araya |
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| Rok vydání: | 2015 |
| Předmět: |
Models
Molecular PTOV1 Chromosomal Proteins Non-Histone Amino Acid Motifs AT-hook GPBP1 Mice Protein structure Tip5 Peptide sequence Cellular localization musculoskeletal system RNA-binding domain Neoplasm Proteins DNA-Binding Proteins Biochemistry Sequence motif Protein Binding Research Paper endocrine system Molecular Sequence Data Biology Binding Competitive parasitic diseases Biomarkers Tumor Animals Humans Amino Acid Sequence Binding site Nucleotide Motifs Molecular Biology Binding Sites Base Sequence RNA Cell Biology DNA HCT116 Cells Molecular biology microscale thermophoresis Protein Structure Tertiary Microscopy Fluorescence Mutation Nucleic acid bacteria Nucleic Acid Conformation protein-RNA interaction sense organs HeLa Cells |
| Zdroj: | RNA Biology |
| ISSN: | 1555-8584 |
| Popis: | The AT-hook has been defined as a DNA binding peptide motif that contains a glycine-arginine-proline (G-R-P) tripeptide core flanked by basic amino acids. Recent reports documented variations in the sequence of AT-hooks and revealed RNA binding activity of some canonical AT-hooks, suggesting a higher structural and functional variability of this protein domain than previously anticipated. Here we describe the discovery and characterization of the extended AT-hook peptide motif (eAT-hook), in which basic amino acids appear symmetrical mainly at a distance of 12-15 amino acids from the G-R-P core. We identified 80 human and 60 mouse eAT-hook proteins and biochemically characterized the eAT-hooks of Tip5/BAZ2A, PTOV1 and GPBP1. Microscale thermophoresis and electrophoretic mobility shift assays reveal the nucleic acid binding features of this peptide motif, and show that eAT-hooks bind RNA with one order of magnitude higher affinity than DNA. In addition, cellular localization studies suggest a role for the N-terminal eAT-hook of PTOV1 in nucleocytoplasmic shuttling. In summary, our findings classify the eAT-hook as a novel nucleic acid binding motif, which potentially mediates various RNA-dependent cellular processes. |
| Databáze: | OpenAIRE |
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