Evaluation of photo-induced cellular damage using photoautotrophic cultures of puk-bung hairy roots as a sensing tool
| Autor: | Masahito Taya, Yoshihiro Ojima, Yuki Tsushima, Masahiro Matsumoto, Motomu Nishioka |
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| Rok vydání: | 2010 |
| Předmět: |
Chlorophyll
DNA Plant Bioengineering Ascorbic Acid Plant Roots Applied Microbiology and Biotechnology Antioxidants Catechin Tissue Culture Techniques chemistry.chemical_compound Chlorogenic acid chemistry.chemical_classification Reactive oxygen species biology food and beverages Light irradiation biology.organism_classification Ascorbic acid Photobiology Oxidative Stress Biochemistry chemistry Spinach Ipomoea Chlorogenic Acid Reactive Oxygen Species DNA DNA Damage Biotechnology |
| Zdroj: | Journal of Bioscience and Bioengineering. 109:392-394 |
| ISSN: | 1389-1723 |
| DOI: | 10.1016/j.jbiosc.2009.09.046 |
| Popis: | In photoautotrophic cultures of pak-bung hairy roots, strong light irradiation (22 W/m2) increased the content of reactive oxygen species (ROS) in the cells, resulting in chlorophyll (Chl) degradation and DNA injury. The Chl degradation rate, RD, can be used as a parameter to measure the cellular damage caused by photo-induced stress. The presence of the antioxidants ascorbic acid, chlorogenic acid or catechin reduced the RD, while lowering the content of ROS and moderating the DNA injury. |
| Databáze: | OpenAIRE |
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