Combination of apoptotic T cell induction and self-peptide administration for therapy of experimental autoimmune encephalomyelitis

Autor: Li Che, Hua Chen, Takashi Maruyama, Dunfang Zhang, Wanjun Chen, Shimpei Kasagi, Lingyun Sun, Jia Li, Hiroko Nakatsukasa, Peter Zanvit, Pin Zhang, Ruiqing Wu, Dandan Wang, Wenwen Jin
Rok vydání: 2019
Předmět:
Zdroj: EBioMedicine. 44:50-59
ISSN: 2352-3964
DOI: 10.1016/j.ebiom.2019.05.005
Popis: BACKGROUND: Clinical trials on multiple sclerosis with repeated injections of monoclonal antibodies depleting CD4(+) T cells have not resulted in much success as a disease therapy. Here, we developed an immunotherapy for EAE in mice by combining a transient depletion of T cells together with the administration of neuron derived peptides. METHODS: EAE was induced in SJL and C57BL/6 mice, by proteolipid protein peptide PLP(139–151) (pPLP) and myelin-oligodendrocyte glycoprotein MOG(35–55) (pMOG) peptides, respectively. Anti-CD4 and anti-CD8 antibody were injected intraperitoneally before or after peptide immunization. EAE scores were evaluated and histology data from brain and spinal cord were analyzed. Splenocytes were isolated and CD4(+), CD4(+)CD25(−) and CD4(+)CD25(+) T cells were purified and cultured in the presence of either specific peptides or anti-CD3 antibody and proliferation of T cells as well as cytokines in supernatant were assessed. FINDINGS: This experimental treatment exhibited therapeutic effects on mice with established EAE in pPLP-susceptible SJL mice and pMOG-susceptible C57BL/6 mice. Mechanistically, we revealed that antibody-induced apoptotic T cells triggered macrophages to produce TGFβ, and together with administered auto-antigenic peptides, generated antigen-specific Foxp3(+) regulatory T cells (T(reg) cells) in vivo. INTERPRETATION: We successfully developed a specific immunotherapy to EAE by generating autoantigen-specific T(reg) cells. These findings have overcome the drawbacks of long and repeated depletion of CD4(+) T cells, but also obtained long-term immune tolerance, which should have clinical implications for the development of a new effective therapy for multiple sclerosis. FUND: This research was supported by the Intramural Research Program of the NIH, NIDCR.
Databáze: OpenAIRE
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